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MABF979 Anti-Bst2 Antibody, clone 44E9

This Anti-Bst2 Antibody, clone 44E9 is validated for use in Flow Cytometry, Western Blotting, Immunocytochemistry, Inhibition for the detection of Bst2.

Anti-Bst2 Antibody, clone 44E9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: Bst2 UniProt Number: Q8R2Q8
MABF979
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HFC, WB, ICC, InhibitionRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF979
DescriptionAnti-Bst2 Antibody, clone 44E9
Alternate Names
  • Bone marrow stromal antigen 2
  • Bst2
  • BST-2
  • HM1.24 antigen
  • CD317
Background InformationBone marrow stromal antigen 2 (UniProt Q8R2Q8; also known as Bst-2, CD317, DAMP-1 protein homolog, HM1.24 antigen, Tetherin) is encoded by the Bst2 (also known as 2310015I10Rik, C87040, GREG) gene (Gene ID 69550) in murine species. Bst-2 is a type II transmembrane glycoprotein that exists as a disulfide bond-linked homodimer on the membrane. In addition, Bst-2 is reported to shuttle between the cell surface and the Golgi apparatus. Bst-2 is expressed in type I IFN-producing cells (IPCs), also known as plasmacytoid dendritic cells (DCs), which function as early responders to viral infections. Bst-2 is also known as tetherin because it prevents the release of enveloped viruses from the host cells by tethering the virions in infected cells. Murine Bst-2 is initially produced with a C-terminal propeptide (a.a. 153-172) sequence, the removal of which yields the mature protein with a large extracellular region (a.a. 52-152), a transmembrane segment (a.a. 31-51), and a short cytoplasmic tail (a.a. 1-30).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ antibody in PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-Bst2 Antibody, clone 44E9 is validated for use in Flow Cytometry, Western Blotting, Immunocytochemistry, Inhibition for the detection of Bst2.
Key Applications
  • Flow Cytometry
  • Western Blotting
  • Immunocytochemistry
  • Inhibition
Application NotesWestern Blotting Analysis: A representative lot detected Bst2 in 50 µg mouse spleen tissue lysate (Courtesy of Dr. Youhai Chen, University of Pennsylvania School of Medicine, USA).
Immunocytochemistry Analysis: A representative lot detected Bst2 immunoreactivity among murine stomal OP9 cells and human embryonic kidney HEK293T cells transformed to express type I INF using retroviral vector MiGr1 (Courtesy of Dr. Youhai Chen, University of Pennsylvania School of Medicine, USA).
Flow Cytometry Analysis: A representative lot detected a high- and a low-Bst2-expressing populations among murine mesenteric lymph node (LN) cells (Courtesy of Dr. Youhai Chen, University of Pennsylvania School of Medicine, USA).
Inhibition Analysis: A representative lot, when administered via tail vein injection (200 µg/mouse), protected mice from death upon Listeria monocytogenes infection (Courtesy of Dr. Youhai Chen, University of Pennsylvania School of Medicine, USA).
Biological Information
ImmunogenRecombinant protein corresponding to the extracellular domain of mouse Bst2.
EpitopeExtracellular domain
Clone44E9
ConcentrationPlease refer to lot specific datasheet.
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Bst2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~19 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in IFN-gamma-treated mouse splenocytes.

Flow Cytometry Analysis: 10 µg of this antibody detected Bst2 in IFN-gamma-treated mouse splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF979 04055977297997