Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

NA20 Anti-BrdU (Ab-2) Mouse mAb (ZBU30)

Anti-BrdU (Ab-2) Mouse mAb (ZBU30) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bromodeoxyuridine

NA20
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
M
Description
OverviewRecognizes BrdU (bromodeoxyuridine). Does not cross-react with thymidine. Use of this antibody requires biosynthetic labeling of the target tissues or cells with the bromodeoxyuridine analog such as 5-Bromo-2′-deoxyuridine (Cat. No. 203806).
Catalogue NumberNA20
Brand Family Calbiochem®
SynonymsAnti-Bromodeoxyuridine
References
ReferencesSchutte, B. et al. 1987. J. Histochem. Cytochem. 35, 1343. Gratzner, H.G. 1982. Science 218, 474. Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385. Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive controlBrdU-labeled cells
Preservative≤0.1% sodium azide
Applications
Key Applications Frozen Sections
Immunofluorescence
Paraffin Sections
Application NotesFlow cytometry (see comments) Frozen Sections (2 µg/ml) Immunofluorescence (see comments) Paraffin Sections (2.5 µg/ml, trypsin pre-treatment required)
Application CommentsUse of this antibody first requires biosynthetic labeling of the target tissues or cells with bromodeoxyuridine (Cat. No. 203806). The DNA must be denatured in order for the BrdU to be detected. This antibody will cross-react with IdU. Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 min. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. This antibody is also reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenBrdU-labeled DNA
CloneZBU30
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NA20 0

Documentation

Anti-BrdU (Ab-2) Mouse mAb (ZBU30) SDS

Title

Safety Data Sheet (SDS) 

Anti-BrdU (Ab-2) Mouse mAb (ZBU30) Certificates of Analysis

TitleLot Number
NA20

References

Reference overview
Schutte, B. et al. 1987. J. Histochem. Cytochem. 35, 1343. Gratzner, H.G. 1982. Science 218, 474. Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385. Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 RFH
SynonymsAnti-Bromodeoxyuridine
ApplicationFlow cytometry (see comments) Frozen Sections (2 µg/ml) Immunofluorescence (see comments) Paraffin Sections (2.5 µg/ml, trypsin pre-treatment required)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 myeloma cells. Recognizes BrdU-labeled DNA.
BackgroundProliferating cells are usually detected by incorporation of [3H]thymidine into DNA and quantitation by autoradiography or scintillation counting. Alternatively, bromodeoxyuridine (BrdU), an analog of thymidine, can be incorporated specifically into the DNA of replicating cells and the extent of incorporation can be detected using antibodies directed against the incorporated analog. The proportion of cells actively proliferating can then be determined by immunofluorescence or flow cytometry as well as immunohistochemistry (which has been done with tumors, tissues, and blood cell elements).
HostMouse
ImmunogenBrdU-labeled DNA
CloneZBU30
IsotypeIgG₁
Speciesa broad range of species
Positive controlBrdU-labeled cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsUse of this antibody first requires biosynthetic labeling of the target tissues or cells with bromodeoxyuridine (Cat. No. 203806). The DNA must be denatured in order for the BrdU to be detected. This antibody will cross-react with IdU. Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 min. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. This antibody is also reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesSchutte, B. et al. 1987. J. Histochem. Cytochem. 35, 1343. Gratzner, H.G. 1982. Science 218, 474. Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385. Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.