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AM04 Anti-Bak (Ab-2) Mouse mAb (TC-102)

Anti-Bak (Ab-2), mouse monoclonal, clone TC-102, recognizes the ~30 kDa Bak protein in A431, HeLa, MCF-7, and SKBR3 cells. It is validated for Western blotting and immunofluorescence.

Anti-Bak (Ab-2) Mouse mAb (TC-102) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bcl-2 Antagonist Killer

AM04
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~30 kDa (apparent MW) Bak protein in A431, HeLa, MCF-7 or SKBR3 cells.
Catalogue NumberAM04
Brand Family Calbiochem®
SynonymsAnti-Bcl-2 Antagonist Killer
References
ReferencesChittenden, T., et al. 1995. Nature 374, 733.
Farrow, S.N., et al. 1995. Nature 374, 731.
Kiefer, M.C., et al. 1995. Nature 736.
Reed, J.C. 1994. J. Cell Biol. 124, 1.
Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative controlSKBR3 cells
Positive controlA431 cells, HeLa cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesImmunoblotting (5 µg/ml)
Immunofluorescence (1 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems for each application.
Biological Information
Immunogenhuman recombinant Bak protein from which the C-terminal and transmembrane domains have been deleted
ImmunogenHuman
Epitopewithin the N-terminal region
CloneTC-102
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AM04 0

Documentation

Anti-Bak (Ab-2) Mouse mAb (TC-102) SDS

Title

Safety Data Sheet (SDS) 

Anti-Bak (Ab-2) Mouse mAb (TC-102) Certificates of Analysis

TitleLot Number
AM04

References

Reference overview
Chittenden, T., et al. 1995. Nature 374, 733.
Farrow, S.N., et al. 1995. Nature 374, 731.
Kiefer, M.C., et al. 1995. Nature 736.
Reed, J.C. 1994. J. Cell Biol. 124, 1.
Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-July-2010 JSW
SynonymsAnti-Bcl-2 Antagonist Killer
ApplicationImmunoblotting (5 µg/ml)
Immunofluorescence (1 µg/ml)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~30 kDa (apparent MW) Bak protein.
BackgroundBak (for bcl-2 homologous antagonist/killer) is a member of the bcl-2 family of proteins. It was independently identified by three different groups using either the two hybrid system for identifying adenovirus E1B 19K interacting proteins or by PCR cloning using degenerate BH1 and BH2 domain primers derived from the human bcl-2 gene. Bak shows a 53% homology with bcl-2 in the BH1 and BH2 domains but an overall homology of 28% with bcl-2. The presence of a hydrophobic tail on the protein suggests that, like bcl-2, it is membrane localized. The bak protein is translated from a 2.2 kb mRNA to yield a 211-216 amino acid protein with a predicted molecular weight of 23.4 kDa, but which migrates by gel electrophoresis as an approximately 30 kDa protein under denaturing conditions. Bak is expressed widely in many tissues including heart, thymus, and colon. The bak gene (bak-1) has been mapped to chromosome 6 and spans approximately 6 kb of DNA3. Southern analysis has indicated the presence of two additional bak genes: bak-2 has 97% homology with bak-1 and maps to chromosome 20, and bak-3, a pseudogene, which maps to chromosome 11. Functionally, bak is similar to bax in that it induces apoptosis. The protein pairs with bcl-xL but unlike its pairing partner has a much more extensive tissue distribution, being present in most tissues tested. While conflicting reports exist for the heterodimerization of bak with other members of the bcl-2 family of proteins, it is clear that bak does partner with the E1B 19K protein.
HostMouse
Immunogen speciesHuman
Immunogenhuman recombinant Bak protein from which the C-terminal and transmembrane domains have been deleted
Epitopewithin the N-terminal region
CloneTC-102
IsotypeIgG2b
Specieshuman, mouse
Positive controlA431 cells, HeLa cells
Negative controlSKBR3 cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems for each application.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesChittenden, T., et al. 1995. Nature 374, 733.
Farrow, S.N., et al. 1995. Nature 374, 731.
Kiefer, M.C., et al. 1995. Nature 736.
Reed, J.C. 1994. J. Cell Biol. 124, 1.
Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.