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MAB3503-I Anti-Apaf-1 Antibody, clone 2E12

This rat monoclonal Anti-Apaf-1 Antibody, clone 2E12, Cat. No. MAB3503-I, has been tested for use in Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting for the detection Apaf-1.

Anti-Apaf-1 Antibody, clone 2E12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: APAF-1 UniProt Number: O14727
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MAB3503-I
100 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IP, EM, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3503-I
DescriptionAnti-Apaf-1 Antibody, clone 2E12
Alternate Names
  • Apoptotic protease-activating factor 1
Background InformationApoptotic protease-activating factor 1 (UniProt: O14727; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 317) in human. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal Anti-Apaf-1 Antibody, clone 2E12, Cat. No. MAB3503-I, has been tested for use in Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting for the detection Apaf-1.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Electron Microscopy
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Hausmann, G., et. al. (2000). J Cell Biol. 149(3):623-34).

Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Moriishi, K., et. al. (1999). Proc Natl Acad Sci USA. 96(17):9683-8).

Immunocytochemistry Analysis: A representative lot detected Apaf-1 in Immunocytochemistry applications (Hausmann, G., et. al. (2000). J Cell Biol. 149(3):623-34).

Immunoprecipitation Analysis: A representative lot detected Apaf-1 in Immunoprecipitation applications (Moriishi, K., et. al. (1999). Proc Natl Acad Sci USA. 96(17):9683-8).

Electron Microscopy Analysis: A representative lot detected Apaf-1 in Electron Microscopy applications (Hausmann, G., et. al. (2000). J Cell Biol. 149(3):623-34).
Biological Information
ImmunogenRecombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
EpitopeN-terminus
Clone2E12
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 2E12 detects Apaf-1 in Jurkat cells. It targets an epitope within first 97 amino acids from the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APAF-1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~140 kDa observed; 141.84 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Apaf-1 in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3503-I 04054839198991