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ABT21 Anti-Amphiphysin II Antibody

Detect Amphiphysin II using this Anti-Amphiphysin II Antibody validated for use in WB.

Anti-Amphiphysin II Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: AMPHL, AMPH2, BIN1, SH3P9 UniProt Number: O00499
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ABT21
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT21
DescriptionAnti-Amphiphysin II Antibody
Alternate Names
  • bridging integrator 1
  • amphiphysin-like
  • box dependant MYC interacting protein 1
  • Amphiphysin II
  • Box-dependent myc-interacting protein 1
  • Amphiphysin-like protein
Background InformationAmphiphysin is a nerve terminal-enriched protein thought to be involved in synaptic vesicle endocytosis. One of three amphiphysin homologs, amphiphysin II may interact with amphiphysin I in order to regulate synaptic vesicle endocytosis. During clathrin-mediated endocytosis, amphiphysin has been reported to bind to a component of the clathrin coat, clathrin assembly protein 2. Mutations in the expression of amphiphysin II may be causal to defects in the endocytosis pathway. Expression of amphiphysin II has been observed in neurons along with some endocrine cell types and spermatocytes.
References
Product Information
FormatAffinity Purified
Control
  • Mouse brain tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Amphiphysin II using this Anti-Amphiphysin II Antibody validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis: 1:1,000 dilution from a previus lot detected Amphiphysin II in 10 µg of rat brain and mouse brain membrane tissue lysates.
Biological Information
ImmunogenKLH-conjugated recombinant protein corresponding to human Amphiphysin II.
EpitopeUnknown
HostRabbit
SpecificityThe antibody is raised against human amphiphysin II, but recognizes both amphiphysin I and Amphiphysin II (see Molecular Weight Notes).
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with mouse and rat. Predicted to react with human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq].
Gene Symbol
  • AMPHL
  • AMPH2
  • BIN1
  • SH3P9
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.

SUBUNIT STRUCTURE: Heterodimer with AMPH. Binds SH3GLB1 By similarity. Interacts (via SH3 domain) with SYNJ1. Interacts (via SH3 domain) with DNM1. Isoform IIA interacts with CLTC. Isoform IIB does not interact with CLTC. Isoform IIC1 does not interact with CLTC. Isoform IIC2 does not interact with CLTC. Interacts with AP2A2. Interacts with AP2B1. Interacts with MYC (via N-terminal transactivation domain); the interaction requires of in a the integrity of the conserved MYC box regions 1 and 2. Interacts with BIN2. Interacts (SH3 domain) with HCV NS5A.

SUBCELLULAR LOCATION: Isoform BIN1: Nucleus.

Isoform IIA: Cytoplasm.

TISSUE SPECIFICTY: Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.

PTM: Phosphorylated by protein kinase C By similarity.

INVOLVEMENT IN DISEASE: Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.

SEQUENCE SIMILARITIES: Contains 1 BAR domain.

Contains 1 SH3 domain.
Molecular WeightIt is well established (Ramjaun, 1997) that both Amphiphysin I and II run substantially slower on SDS-PAGE with a larger apparent molecular weight than the predicted molecular weights.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Amphiphysin II in 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT21 04053252650475