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ST1088 Anti-Akt, PH Domain Mouse mAb (SKB1)

Anti-Akt, PH Domain, mouse monoclonal, clone SKB1, recognizes the ~60 kDa Akt protein in A431 cells. It is validated for flow cytometry, Western blotting, and immunoprecipitation.

Anti-Akt, PH Domain Mouse mAb (SKB1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-PKB, Pleckstrin Homology Domain

ST1088
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1088-1SET Plastic ampoule 1 set
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Description
OverviewRecognizes the ~60 kDa Akt protein in A431 cells. Useful for the concentration of Akt in samples by immunoprecipitation prior to use in the K-LISA™ Akt Activity Kit (Cat. No. CBA019).
  • Antibody Target Gene Symbol: AKT1
  • Target Synonym: AKT, MGC99656, PKB, PKB-ALPHA, PKB/AKT, PRKBA, Protein kinase B, RAC, RAC-ALPHA, Thymoma viral proto-oncogene 1
  • Entrez Gene Name: v-akt murine thymoma viral oncogene homolog 1
  • Hu Entrez ID: 207 (Related Antibodies: 530311, PK1203, PC510 and 124001)
  • Mu Entrez ID: 11651
  • Rat Entrez ID: 24185
    		•
    Catalogue NumberST1088
    Brand Family Calbiochem®
    SynonymsAnti-PKB, Pleckstrin Homology Domain
    Application Data
    Detection of human Akt by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-Akt, PH Domain Mouse mAb (SKB1) (Cat. No. ST1088) (0.5 µg/ml). Detection: chemiluminescence.
    References
    ReferencesBellacosa, A., et al. 1998. Oncogene 17, 313.
    Product Information
    FormLiquid
    Formulation100 µg antibody in 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4 and 100 µg control lysate.
    Positive controlA431 cells (included)
    Preservative≤0.1% sodium azide
    Quality LevelMQ100
    Applications
    Application ReferencesImmunoblotting Bourguignon, L.Y.W., et al. 2003. J. Biol. Chem. 278, 29420. Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958. Immunoprecipitation, Active Enzyme Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958.
    Key Applications Flow Cytometry
    Immunoblotting (Western Blotting)
    Immunoprecipitation
    Application NotesFlow Cytometry (see comments)
    Immunoblotting (0.5-2 µg/ml, see application references)
    Immunoprecipitation (4 µg/0.5 mg total protein; see comments and see application references)
    Application CommentsThis antibody will immunoprecipitate active Akt from cell lysates. Useful for immunoprecipitation of Akt prior to activity assay using the K-LISA™ Akt Activity Kit (Cat. No. CBA019). This antibody has also been reported to work for flow cytometry applications. Antibody should be titrated for optimal results in individual systems.
    Biological Information
    Immunogena recombinant protein consisting of amino acids 1-149 of human Akt 1 fused to GST
    ImmunogenHuman
    CloneSKB1
    HostMouse
    IsotypeIgG
    Species Reactivity
    • Human
    • Mouse
    • Rat
    Antibody TypeMonoclonal Antibody
    Concentration Label Please refer to vial label for lot-specific concentration
    Storage and Shipping Information
    Ship Code Shipped with Blue Ice or with Dry Ice
    Toxicity Standard Handling
    Storage -20°C
    Avoid freeze/thaw Avoid freeze/thaw
    Do not freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Global Trade Item Number
    Catalogue Number GTIN
    ST1088-1SET 04055977224412