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ABT545-25UL Anti-Aggrecan

Anti-Aggrecan, Cat. No. ABT545, is a rabbit polyclonal antibody that detects Aggrecan core protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

ABT545-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABT545-25UL
DescriptionAnti-Aggrecan
Alternate Names
  • Aggrecan core protein
  • Cartilage-specific proteoglycan core protein
  • CSPCP
Background InformationAggrecan core protein (UniProt: Q61282; also known as Cartilage-specific proteoglycan core protein, CSPCP) is encoded by the Acan (also known as Agc, Agc1) gene (Gene ID: 11595) in murine species. Aggrecan, a secreted proteoglycan, is a major component of extracellular matrix of cartilagenous tissues. Its major function is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region and may play a regulatory role in the matrix assembly of the cartilage. Aggrecan contains two globular domains, G1 and G2 that comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. The G1 region is located in the N-terminus of the core protein and interacts with hyaluronic acid (HA). G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. The G2 region is composed of two B-like domains, but does not have the ability to interact with HA. G2 region is separated from the G1 region by the interglobular domain, which is a prominent site for proteolysis. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3. Aggrecan is present in the extracellular matrix in the form of proteoglycan aggregates, in which many aggrecan molecules interact with hyaluronan and a link protein stabilizes each interaction. It is reported that aggrecan structure changes throughout the life cycle due to changes in synthetic and degradative events. Changes due to synthesis alter the structure of the chondroitin sulfate and keratan sulfate chains, whereas those due to degradation cause cleavage of all components of the aggregate. Aggrecan is also present in most of the retina including the outer nuclear layer, the outer plexiform layer, and the inner plexiform layer.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Aggrecan, Cat. No. ABT545, is a rabbit polyclonal antibody that detects Aggrecan core protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Aggrecan in mouse cartilage tissue sections.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal half of murine Aggrecan core protein.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityAnti-Aggrecan is a rabbit polyclonal antibody that detects Aggrecan core protein. It targets an epitope within 150 amino acids from the C-terminal half.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Acan
  • Agc
  • Agc1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~270 kDa observed; 221.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human Chondrocytes.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Aggrecan in lysate from human chondrocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT545-25UL 04054839028960