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DP08 Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10)

This Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) is validated for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation for the detection of Adenovirus 5 E1B 55 kDa.

Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
DP08
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Adenovirus Infected CellsRMonoclonal Antibody
Description
OverviewRecognizes the ~19 kDa 2 E1B and the ~55 kDa 5 E1B adenovirus proteins in HEK293 cells. Will also immunoprecipitate associated proteins such as p53 and cyclins.
Catalogue NumberDP08
Brand Family Calbiochem®
Application Data
Detection of adenovirus 5 E1B 55 kDa protein by immunocytochemistry. Sample: HEK293 cells fixed with methanol. Primary antibody: Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) (Cat. No. DP08) (4 µg/ml). Detection: fluorescence (FITC).
References
ReferencesWhyte, P., et al. 1988. Nature 334, 124.
Harlow, E., et al. 1986. J. Virol. 39, 861.
Ziff, E. and Velcich, A. 1985. Cell 40, 705.
Houweling, A., et al. 1980. Virology 105, 537.
Berk, A.J., et al. 1979. Cell 17, 935.
Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
Shiroki, K., et al. 1979. Virology 95, 127.
Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
Graham, F.L., et al. 1974. Biol. 39, 637.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controlHS27 cells
Positive control293 cells
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmunocytochemistry and Immunofluoresecence Zantema, A., et al. 1985. Virology 142, 44.
Key Applications Immunofluorescence
Immunoprecipitation
Application NotesImmunocytochemistry (4 µg/ml, see application references)
Immunofluorescence (4 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample)
Application CommentsThis antibody will immunoprecipitate adenovirus 2 and 5 E18 proteins and associated proteins (such as Rb and cyclins) as well as stain infected cells by immunofluorescence and immunoperoxidase. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenadenovirus 5 E1B protein
Clone9C10
HostRat
IsotypeIgG2b
Species Reactivity
  • Adenovirus Infected Cells
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DP08 0

Documentation

Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) Certificates of Analysis

TitleLot Number
DP08

References

Reference overview
Whyte, P., et al. 1988. Nature 334, 124.
Harlow, E., et al. 1986. J. Virol. 39, 861.
Ziff, E. and Velcich, A. 1985. Cell 40, 705.
Houweling, A., et al. 1980. Virology 105, 537.
Berk, A.J., et al. 1979. Cell 17, 935.
Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
Shiroki, K., et al. 1979. Virology 95, 127.
Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
Graham, F.L., et al. 1974. Biol. 39, 637.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
ApplicationImmunocytochemistry (4 µg/ml, see application references)
Immunofluorescence (4 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample)
Application Data
Detection of adenovirus 5 E1B 55 kDa protein by immunocytochemistry. Sample: HEK293 cells fixed with methanol. Primary antibody: Anti-Adenovirus 5 E1B 55 kDa Rat mAb (9C10) (Cat. No. DP08) (4 µg/ml). Detection: fluorescence (FITC).
DescriptionPurified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with YO rat myeloma cells. Recognizes the ~19 kDa 2 E1B and the ~55 kDa adenovirus 5 E1B proteins.
BackgroundThe early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the leftmost 11% of the viral genome, and consists of two transcriptional units, E1A and E1B. Region E1A is sufficient for partial transformation and immortalization of primary cells, whereas the E1B function is normally required for complete transformation. In addition to their essential role in transformation, E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection, and are able to either activate or repress the transcription of specific cellular gene. E1A oncogene proteins form specific complexes with cellular proteins including the anti-oncogene retinoblastoma susceptibility gene product, p105.
HostRat
Immunogenadenovirus 5 E1B protein
Clone9C10
IsotypeIgG2b
Speciesadenovirus infected cells
Positive control293 cells
Negative controlHS27 cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody will immunoprecipitate adenovirus 2 and 5 E18 proteins and associated proteins (such as Rb and cyclins) as well as stain infected cells by immunofluorescence and immunoperoxidase. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesWhyte, P., et al. 1988. Nature 334, 124.
Harlow, E., et al. 1986. J. Virol. 39, 861.
Ziff, E. and Velcich, A. 1985. Cell 40, 705.
Houweling, A., et al. 1980. Virology 105, 537.
Berk, A.J., et al. 1979. Cell 17, 935.
Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
Shiroki, K., et al. 1979. Virology 95, 127.
Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
Graham, F.L., et al. 1974. Biol. 39, 637.
Application referencesImmunocytochemistry and Immunofluoresecence Zantema, A., et al. 1985. Virology 142, 44.