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AB9596-200UL Anti-ASIC4 Antibody

Anti-ASIC4 Antibody detects level of ASIC4 & has been published & validated for use in WB.

Anti-ASIC4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters, Neuroinflammation & Pain Gene Symbol: ACCN4, MGC24860, ASIC4, BNAC4, MGC17248 UniProt Number: Q96FT7
AB9596-200UL
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB9596-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-ASIC4 Antibody
References
Product Information
FormatAffinity Purified
Control
  • Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationAnti-ASIC4 Antibody detects level of ASIC4 & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern blot: 1:200-1:500 using ECL on rat DRG lysate, rat brain and the ND7/23 cell line.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.SUGGESTED WESTERN BLOT PROTOCOL



1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.

2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.

3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)

4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).

5. Destain with deionized water.

6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for

10 min, and filtered through glass filter (Gelman Acrodisc).

7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.

8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.

9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.

10. Wash 4 x 10 min. with PBS-0.1% tween 20.

11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600).
Biological Information
ImmunogenPurified peptide from amino acids 7-16 of rat ASIC4 (Accession number Q9QYV9).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes ASIC4 (H+ gated cation channel, SPASIC, ACCN4).
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. This gene is predominantly expressed in the pituitary gland, and was considered a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder, however, no correlation was found between mutations in this gene and PDC. Alternative splicing at this locus results in two transcript variants encoding different isoforms.
Gene Symbol
  • ACCN4
  • MGC24860
  • ASIC4
  • BNAC4
  • MGC17248
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q96FT7 # Probable cation channel with high affinity for sodium.
SIZE: 647 amino acids; 70033 Da
SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Expressed in pituitary gland. Weakly expressed in brain, vestibular system and organ of Corti.
SIMILARITY: SwissProt: Q96FT7 ## Belongs to the amiloride-sensitive sodium channel family.
MISCELLANEOUS: In vitro, has no proton-gated channel activity.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB9596-200UL 04053252582912