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05-1427 Anti-ARF1 Antibody, clone 3F1

Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).

Anti-ARF1 Antibody, clone 3F1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: ARF1 UniProt Number: P84077
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05-1427
100 µg  
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Overview

Replacement Information
Replacement Information05-1427 is a recommended replacement for MAB3779

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ELISA, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue Number05-1427
ReplacesMAB3779
DescriptionAnti-ARF1 Antibody, clone 3F1
Alternate Names
  • ADP-ribosylation factor 1
Background InformationARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).
Key Applications
  • Western Blotting
  • ELISA
  • Immunohistochemistry (Paraffin)
Application NotesELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer
Biological Information
ImmunogenSynthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.
EpitopeC-terminus
Clone3F1
HostMouse
SpecificityRecognizes human ARF1
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • ARF1
Purification MethodPurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P84077 # GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
SIZE: 181 amino acids; 20697 Da
SUBUNIT: Interacts with PRKCABP, GGA1, DDEF2 and PIP5K1A (By similarity). Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Binds DDEF2. Interacts with ARHGAP21.
SIMILARITY: SwissProt: P84077 ## Belongs to the small GTPase superfamily. Arf family
Molecular Weight~21 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-1427 04053252277665

Documentation

Anti-ARF1 Antibody, clone 3F1 SDS

Title

Safety Data Sheet (SDS) 

Anti-ARF1 Antibody, clone 3F1 Certificates of Analysis

TitleLot Number
Anti-ARF1, clone 3F1 - NG1590610 NG1590610
Anti-ARF1, clone 3F1 - NG1590617 NG1590617
Anti-ARF1, clone 3F1, C-terminus - 2116005 2116005
Anti-ARF1, clone 3F1, C-terminus - 2128248 2128248
Anti-ARF1, clone 3F1, C-terminus - 2153064 2153064
Anti-ARF1, clone 3F1, C-terminus - 2272153 2272153
Anti-ARF1, clone 3F1, C-terminus - 3427188 3427188
Anti-ARF1, clone 3F1, C-terminus - NG1726950 NG1726950
Anti-ARF1, clone 3F1, C-terminus - NG1943280 NG1943280
Anti-ARF1, clone 3F1, C-terminus -2557580 2557580
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