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MAB348-100UL Anti-APP A4 Antibody, a.a. 66-81 of APP (NT), clone 22C11

Anti-APP A4 Antibody, a.a. 66-81 of APP (N-terminus), clone 22C11 detects level of Alzheimer Precursor Protein A4 & has been published & validated for use in IF, IH, IH(P) & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: APP, PN-II, AD1, ABETA, CTFgamma, APPI, ABPP, A4, CVAP, PN2, PreA4, AAA UniProt Number: P05067
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MAB348-100UL
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, Mk, Po, FIF, IHC, IH(P), WBMAscitesMonoclonal Antibody
Description
Catalogue NumberMAB348-100UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-APP A4 Antibody, a.a. 66-81 of APP (NT), clone 22C11
Alternate Names
  • APP
Background InformationAmyloid Precursor Protein (APP) is expressed in the brain, kidney, heart, and spleen of fetal tissues; it is induced during neuronal differentiation. The most-substantiated role for APP is in synaptic formation and repair; its expression is upregulated during neuronal differentiation and after neural injury. In adult brain, highest expression of APP is found in the frontal lobe of the cortex. Moderate expression is observed in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimer's disease (AD). Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humans and in cases of Alzheimer's disease. The principle component of this extracellular amyloid is beta/A4, a 4 kDa peptide derived from the larger APP. The creation, transport and function of these proteins are currently under investigation.
References
Product Information
FormatAscites
Control
  • Brain tissue.
PresentationUnpurified ascites mouse monoclonal IgG1 containing no preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-APP A4 Antibody, a.a. 66-81 of APP (N-terminus), clone 22C11 detects level of Alzheimer Precursor Protein A4 & has been published & validated for use in IF, IH, IH(P) & WB.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry:
Previous lot tested Immunohistochemistry on 4% paraformaldehyde/15% picric acid perfused and fixed, frozen and paraffin embedded sections.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPurified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.
Epitopea.a. 66-81 of APP (N-terminus)
Clone22C11
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with pre-A4. The antibody recognizes a N-terminal epitope on the pre-A4 molecule
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Monkey
  • Pig
  • Fish
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
Gene Symbol
  • APP
  • PN-II
  • AD1
  • ABETA
  • CTFgamma
  • APPI
  • ABPP
  • A4
  • CVAP
  • PN2
  • PreA4
  • AAA
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P05067 # The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.
SIZE: 770 amino acids; 86943 Da
SUBUNIT: Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, MAPK8IP1, SHC1 and, Numb and Dab1 (By similarity). Binding to Dab1 inhibits its serine phosphorylation (By similarity). Also interacts with GPCR-like protein BPP, FPRL1, APPBP1, IB1, KNS2 (via its TPR domains) (By similarity), APPBP2 (via BaSS) and DDB1. In vitro, it binds MAPT via the MT-binding domains (By similarity). Associates with microtubules in the presence of ATP and in a kinesin-dependent manner (By similarity). Interacts, through a C-terminal domain, with GNAO1. Amyloid beta-42 binds CHRNA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Interacts with CPEB1 (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Note=Cell surface protein that rapidly becomes internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O- glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C- terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. Gamma-CTF(59) peptide is located to both the cytoplasm and nuclei of neurons. It can be translocated to the nucleus through association with Fe65. Beta- APP42 associates with FRPL1 at the cell surface and the complex is then rapidly internalized. APP sorts to the basolateral surface in epithelial cells. During neuronal differentiation, the Thr-743 phosphorylated form is located mainly in growth cones, moderately in neurites and sparingly in the cell body. Casein kinase phosphorylation can occur either at the cell surface or within a post-Golgi compartment.
TISSUE SPECIFICITY: Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes.
DOMAIN: SwissProt: P05067 The basolateral sorting signal (BaSS) is required for sorting of membrane proteins to the basolateral surface of epithelial cells. & The NPXY sequence motif found in many tyrosine- phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C- terminal to the NPXY motif are often required for complete interaction. The PID domain-containing proteins which bind APP require the YENPTY motif for full interaction. These interactions are independent of phosphorylation on the terminal tyrosine residue. The NPXY site is also involved in clathrin-mediated endocytosis.
PTM: Proteolytically processed under normal cellular conditions. Cleavage by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is non-amyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59). & Proteolytically cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. & N- and O-glycosylated. O-linkage of chondroitin sulfate to the L-APP isoforms produces the APP proteoglycan core proteins, the appicans. The chondroitin sulfate chain of appicans contains 4-O-sulfated galactose in the linkage region and chondroitin sulfate E in the repeated disaccharide region (By similarity). & Phosphorylation in the C-terminal on tyrosine, threonine and serine residues is neuron-specific. Phosphorylation can affect APP processing, neuronal differentiation and interaction with other proteins. Phosphorylated on Thr-743 in neuronal cells by Cdc5 kinase and Mapk10, in dividing cells by Cdc2 kinase in a cell- cycle dependent manner with maximal levels at the G2/M phase and, in vitro, by GSK-3-beta. The Thr-743 phosphorylated form causes a conformational change which reduces binding of Fe65 family members. Phosphorylation on Tyr-757 is required for SHC binding. Phosphorylated in the extracellular domain by casein kinases on both soluble and membrane-bound APP. This phosphorylation is inhibited by heparin. & Extracellular binding and reduction of copper, results in a corresponding oxidation of Cys-144 and Cys-158, and the formation of a disulfide bond. In vitro, the APP-Cu(+) complex in the presence of hydrogen peroxide results in an increased production of beta-amyloid-containing peptides.
DISEASE: SwissProt: P05067 # Defects in APP are a cause of autosomal dominant Alzheimer disease (AD) [MIM:104300]. AD is the most prevelant form of dementia, characterized by neurofibrillary tangles and amyloid plaques deposition in the brain. Identical lesions occur in the neurons of aged Down syndrome but at an earlier age than in AD. The major constituent of these neuritic plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. Mutations occurring at the beta-amyloid N-terminal, such as the Swedish double mutation, appear to increase levels of beta-amyloid by facilitating beta-secretase cleavage resulting in elevated levels of both beta-APP42 and beta-APP40. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31, are also implicated in AD neuronal death. Alzheimer disease caused by mutations in APP is a rare occurrence and usually causes the familial or early-onset form of the disease (FAD). Flemish-type AD is characterized by, in addition to presenile dementia, cerebral hemorrhaging due to cerebral amyloid angiopathy which is similar to, but distinct from, cerebroarterial amyloidosis Dutch type. Only about 5% of all cases of Alzheimer disease are caused by FAD mutations, the rest are sporadic. & Defects in APP are the cause of hereditary cerebral hemorrhage with amyloidosis Dutch type (HCHWAD) [MIM:609065]. HCHWAD is characterized by amyloid deposits in cerebral vessels. The principal clinical characteristics are recurring cerebral hemorrhages, sometimes preceded by migrainous headaches or mental cleavage. Beta-APP40 is the predominant form of cerebrovascular amyloid. & Defects in APP are the cause of hereditary cerebroarterial amyloidosis Iowa type [MIM:605714]. Hereditary cerebroarterial amyloidosis Iowa type is an autosomal dominant dementia beginning in the sixth or seventh decade of life. The patients have progressive aphasic dementia, leukoencephalopathy, and occipital calcifications. They do not present cerebral hemorrhaging.
SIMILARITY: Belongs to the APP family. & Contains 1 BPTI/Kunitz inhibitor domain.
MISCELLANEOUS: Chelation of metal ions, notably copper, iron and zinc, can induce histidine-bridging between beta-amyloid molecules resulting in beta-amyloid-metal aggregates. The affinity for copper is much higher than for other transient metals and is increased under acidic conditions. Extracellular zinc-binding increases binding of heparin to APP and inhibits collagen-binding.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by immunohistochemistry(paraffin) on Alzheimer’s Disease-Hypothalamus Tissue.

Immunohistochemistry(paraffin):
Amyloid (β-A4-protein, cat. # MAB348) staining on Alzheimer’s Disease-Hypothalamus. Tissue pretreated with Citrate, pH 6.0. The antibody was diluted to 1:80, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as staining on plaque deposits (dark brown) or as cerebral vascular amyloid deposition in the walls of blood vessels (brown).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB348-100UL 04053252664571

Documentation

Anti-APP A4 Antibody, a.a. 66-81 of APP (NT), clone 22C11 SDS

Title

Safety Data Sheet (SDS) 

Anti-APP A4 Antibody, a.a. 66-81 of APP (NT), clone 22C11 Certificates of Analysis

TitleLot Number
Anti-Alzheimer Precursor Protein A4, -2793862 2793862
Anti-Alzheimer Precursor Protein A4, a.a. 66-81 of APP (N-terminus), clone 22C11 - 2366557 2366557
Anti-Alzheimer Precursor Protein A4, a.a. 66-81 of APP (N-terminus), clone 22C11 - 2452530 2452530
Anti-Alzheimer Precursor Protein A4, a.a. 66-81 of APP (N-terminus), -2556922 2556922
Anti-Alzheimer Precursor Protein A4, a.a. 66-81 of APP (N-terminus), -2624964 2624964
Anti-Alzheimer Precursor Protein A4, a.a. 66-81 of APP (N-terminus), -2633750 2633750
Anti-Alzheimer Precursor Protein A4, a.a. 66-81 of APP (N-terminus), -2658930 2658930
Anti-Alzheimer Precursor Protein A4, a.a. 66-81 of APP (N-terminus), -2697568 2697568
Anti-Alzheimer Precursor Protein A4, a.a. 66-81 of APP (N-terminus), -2726804 2726804
Anti-Alzheimer Precursor Protein A4, a.a. 66-81 of APP (N-terminus), -2746403 2746403
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References

Reference overviewPub Med ID
Comparison of myelin, axon, lipid, and immunopathology in the central nervous system of differentially myelin-compromised mutant mice: a morphological and biochemical study.
Loers, Gabriele, et al.
Mol. Cell. Neurosci., 27: 175-89 (2004)  2004

15485773 15485773

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Catalogue Number Description
MAB348 Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11

Product Families

Amyloid Precursor Protein Antibodies

APP is the precursor molecule whose proteolysis generates beta amyloid (Aβ), a 37 to 49 amino acid peptide that is the primary component of amyloid plaques found in Alzheimer's disease patients.
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies