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ABS571 Anti-3-PGDH Antibody

This Anti-3-PGDH Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of 3-PGDH.

Anti-3-PGDH Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: PHGDH, PGDH3 UniProt Number: O43175
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ABS571
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS571
DescriptionAnti-3-PGDH Antibody
Alternate Names
  • D-3-phosphoglycerate dehydrogenase
  • 3-PGDH
Background Information3-PGDH (PHGDH) protein in an important enzyme in L-serine biosynthesis. 3-PGDH performs the first rate limiting step in the phosphorylated pathway of serine biosynthesis. 3-PGDH is also a biomarker in certain cancers that over express 3-PGDH. Moreover, 3-PGDH is the essential enzyme needed for cells to traverse the S phase and divide, and it plays a key role in T cell activation and proliferation for instance. Interestingly, even though 3-PGDH knockdown and reduced serine synthesis slows growth and proliferation of many cancer cells in vitro 3-PGDH knockdown does not affect tumor maintenance and growth in established breast cancer xenograft models, suggesting that 3-PGDH-dependent cancer cell growth may be context-dependent.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-3-PGDH Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of 3-PGDH.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected 3-PGDH in human cerebral cortex, human kidney, and human cerebellum tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human 3-PGDH near the N-terminus.
EpitopeNear N-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Porcine, Chimpanzee based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PHGDH
  • PGDH3
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~54 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected 3-PGDH in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS571 04055977073287