Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
Catalogue Number
565834
Brand Family
Calbiochem®
References
References
Hoang, et al. 1996. J. Biol. Chem.271, 26131.
Leyns, et al. 1997 Cell88, 747.
Wang, et al. 1997 Cell88, 757.
Mayr, et al. 1997 Mech. Dev.63, 109.
Rattner, et al. 1997. PNAS94, 2859.
Proliferation Assay: ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA: 50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
Purity
≥95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotxin: ≤1 EU/µg
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
sFRP-3, His•Tag®, Mouse, Recombinant Certificates of Analysis
Title
Lot Number
565834
References
Reference overview
Hoang, et al. 1996. J. Biol. Chem.271, 26131.
Leyns, et al. 1997 Cell88, 747.
Wang, et al. 1997 Cell88, 757.
Mayr, et al. 1997 Mech. Dev.63, 109.
Rattner, et al. 1997. PNAS94, 2859.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
21-Sepmtember-2011 RFH
Description
Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
Form
Lyophilized
Formulation
Lyophilized from 0.2 µm filtered PBS, BSA.
Purity
≥95% by SDS-PAGE
Contaminants
Endotxin: ≤1 EU/µg
Biological activity
Proliferation Assay: ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA: 50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
Solubility
Reconstitute with sterile PBS contanining ≥1% human or bovine serum albumin. Please refer to the vial label for lot-specific reconstitution volume.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Hoang, et al. 1996. J. Biol. Chem.271, 26131.
Leyns, et al. 1997 Cell88, 747.
Wang, et al. 1997 Cell88, 757.
Mayr, et al. 1997 Mech. Dev.63, 109.
Rattner, et al. 1997. PNAS94, 2859.
