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69418 pET-3a DNA - Novagen

69418
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69418-3 Plastic ampoule 10 μg
Description
OverviewThe pET-3a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. These vectors are the precursors to many pET family vectors; the pET-23a-d(+) series corresponds to pET-3a-d but incorporates several additional features. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB026).

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69418
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69418-3 07790788052577

Documentation

pET-3a DNA - Novagen Certificates of Analysis

TitleLot Number
69418

Citations

Title
  • Amanda Nga-Sze Mak, et al. (2007) Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site. Nucleic Acids Research 35, 6259-6267.
  • Line Johnsen, et al. (2005) 1.6 Å crystal structure of EntA-Im: A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A. Journal of Biological Chemistry 280, 19045-19050.
  • Fabrice A. C. Klein, et al. (2005) Biochemical and NMR mapping of the interface between CREB-binding protein and ligand binding domains of nuclear receptor: beyond the LXXLL motif. Journal of Biological Chemistry 280, 5682-5692.
  • Orbán Komonyi, et al. (2005) DTL, the Drosophila homolog of PIMT/Tgs1 nuclear receptor coactivator interacting protein/RNA methyltransferase, has an essential role in development. Journal of Biological Chemistry 280, 12397-12404.
  • Astrid Krmpotic, et al. (2005) NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145. journal of Experimental Medicine 201, 211-220.
  • James T. Macdonald, et al. (2005) Unfolding crystallins: The destabilizing role of a β-hairpin cysteine in βB2-crystallin by simulation and experiment. Protein Science 14, 1282-1292.
  • Evelyne Raux-Deery, et al. (2005) Identification and characterization of the terminal enzyme of siroheme biosynthesis from Arabidopsis thaliana: a plastid-located sirohydrochlorin ferrochelatase containing a 2Fe-2S center. Journal of Biological Chemistry 280, (4713- 4721).
  • Barbara Zambelli, et al. (2005) UreG, a chaperone in the urease assembly process, is an intrinsically unstructured GTPase that specifically binds Zn2+. Journal of Biological Chemistry 280, 4684-4695.
  • Dan Gu, et al. (2004) Identification and characterization of the DNA-binding domain of the multifunctional PutA flavoenzyme. Journal of Biological Chemistry 279, 31171-31176.
  • Liliya A. Yatsunyk, Benjamin E. Ramirez and Amy C. Rosenzweig. (2004) Yeast Cox17 solution structure and copper(I) binding. Journal of Biological Chemistry 279, 53584-53592.
  • Claudia Naumann, Thomas Hartmann and Dietrich Ober. (2002) Evolutionary recruitment of a flavin-dependent monooxygenase for the detoxification of host plant-acquired pyrrolizidine alkaloids in the alkaloid-defended arctiid moth Tyria jacobaeae. Procedings of the National Academy of Science 99, 6085-6090.
  • Luca Pellegrini, et al. (2000) Crystal structure of fibroblast growth factor receptor ectodomain bound to ligand and heparin. 407, 1029-1034.
  • Kaare Teilum, Lars Ostergaard and Kearen Welinder. (1999) Disulfide bond formation and folding of plant peroxidases expressed as inclusion body proteins in Escherichia coli thioredoxin reductase negative strains. Protein Expression and Purification 15, 77-82.
  • F.-X. Gomis-Ruth, et al. (1997) Mechanism of inhibition of the human matrix metalloproteinase stromelysin-1 by TIMP-1. 389, 77-81.
  • R. Koljak, et al. (1997) Identification of a naturally occurring peroxidase-lipooxygenase fusion protein. Science 277, 1994-1996.
  • Weibiao Huang and Erik Bateman. (1995) Cloning, expression, and characterization of the TATA-binding protein (TBP) promoter binding factor, a transcription activator of the Acanthamoeba TBP gene. Journal of Biological Chemistry 270, 28839-28847.
  • User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB026VM pET-3a-d Vector Map

    Vector Sequence

    Title
    pET-3a Vector Sequence

    Related Products & Applications

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    Categories

    Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors