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14-925 Wee1 Protein, active, 10 µg

Active, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.

Wee1 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-925
10 µg  Also available in 250μg size as catalog # 14-925M and in bulk as catalog # 14-925-K.
Purchase on Sigma-Aldrich

Overview

Description
Catalogue Number14-925
Trade Name
  • Upstate
DescriptionWee1 Protein, active, 10 µg
OverviewActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Product Information
Presentation50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Quality LevelMQ100
Applications
ApplicationActive, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceN-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • WEE1
Protein TargetWEE1
Purification MethodPurified using glutathione agarose.
Target Sub-FamilyCK1
UniProt Number
Molecular Weight77 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size as catalog # 14-925M and in bulk as catalog # 14-925-K.
Global Trade Item Number
Catalogue Number GTIN
14-925 04053252832147