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SCC210 UFH-001 Human Breast Cancer Cell Line

The UFH-001 triple negative CAIX+ human breast cancer cell line is suitable as a model for studying the mechanisms of triple negative breast cancer progression, metastasis and migration.

SCC210
≥1X10⁶ cells/vial   
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Overview

Replacement Information
Description
Catalogue NumberSCC210
DescriptionUFH-001 Human Breast Cancer Cell Line
OverviewThe UFH-001 triple negative CAIX+ human breast cancer cell line constitutively expresses CAIX under both hypoxic and normoxic conditions, exhibits an aggressive TNBC phenotype in vivo, and supports tumor growth in a mouse xenograft model (2). The constitutive expression of CAIX in UFH-001 cells facilitates its evaluation as a potential therapeutic target both in vitro and in vivo.

Source:
UFH-001 cells are derived from MCF-10A cells, a spontaneously immortalized breast cancer epithelial cell line originating from breast tissue of a healthy female patient (3). UFH-001 cells were sorted for CAIX expression by flow cytometry (2).
Background InformationTriple negative breast cancer (TNBC) accounts for 15-20% of breast cancer cases and is characterized by the absence of estrogen receptor (ER), progesterone receptor (PR), and human EGF receptor 2 (HER2) (1). TNBC is typically more aggressive and difficult to treat than more common types of breast cancer and is more likely to recur after treatment. Carbonic anhydrase IX (CAIX) catalyzes the reversible hydration of carbon dioxide and is a marker for hypoxic regions of breast tumors. CAIX expression is associated with poor prognosis in TNBC and hormone-resistant breast tumors.

References:
1. Sporikova Z, et al., (2018) Clin Breast Cancer 18(5): e841-e850.
2. Chen Z et al., (2018) Cancer Biol Ther 19(7): 598-608.
3. Soule HD et al., (1990) Cancer Res 50(18): 6075-6086.
References
Product Information
Applications
ApplicationThe UFH-001 triple negative CAIX+ human breast cancer cell line is suitable as a model for studying the mechanisms of triple negative breast cancer progression, metastasis and migration.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC210 04054839736148