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14-479M Rsk1/MAPKAP Kinase 1a Protein, active, Rat, 250 µg

Active, recombinant full-length rat RSK1 (residues 1-end), containing additional N-terminal His6 & HA (YPYDV PDYA) tags, for use in Kinase Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-479M
250 µg  Also available in 10 μg size (cat#14-479) and in bulk (cat# 14-479-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-479M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionRsk1/MAPKAP Kinase 1a Protein, active, Rat, 250 µg
OverviewRecombinant full-length rat RSK1 (residues 1-end), containing additional Nterminal His6 and HA (YPYDV PDYA) tags
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, recombinant full-length rat RSK1 (residues 1-end), containing additional N-terminal His6 & HA (YPYDV PDYA) tags, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed in baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene Symbol
  • RPS6KA1
  • HU-1
  • RSK-1
  • MAPKAPK1A
  • RSK
  • pp90RSK1
  • p90S6K
  • RSK1
Protein TargetRsk1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q15418 # Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
COFACTOR: Magnesium.
SIZE: 735 amino acids; 82723 Da
SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation.
PTM: Autophosphorylated on Ser-380, as part of the activation process.
SIMILARITY: SwissProt: Q15418 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains.
Molecular Weight88kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of the MAPKAP Kinase 2 substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-479) and in bulk (cat# 14-479-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-479M 04053252512407