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539735 PTP1B, Human, Recombinant, E. coli

PTP1B, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Tyrosine Phosphatase 1B

539735
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
539735-50UG Plastic ampoule 50 μg
Description
OverviewRecombinant, human PTP1B expressed in E. coli. PTP1B, an ubiquitous nontransmembrane protein tyrosine phosphatase, was originally identified in human placenta. This highly active enzyme is useful for the study of tyrosine phosphatase kinetics, substrate specificity, and for screening inhibitors.
Catalogue Number539735
Brand Family Calbiochem®
SynonymsProtein Tyrosine Phosphatase 1B
References
ReferencesPuius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420.
Liu, F., et al. 1996. J. Biol. Chem. 271, 31290.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphopeptide substrate (Cat. No. 539737) per min at 30°C, pH 7.2 using 150 µM of substrate.
FormLiquid
FormulationIn 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.05% NP-40, pH 7.2.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥20 units/mg
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539735-50UG 04055977194289

Documentation

PTP1B, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

PTP1B, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
539735

References

Reference overview
Puius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420.
Liu, F., et al. 1996. J. Biol. Chem. 271, 31290.

Brochure

Title
Protein Phosphatases Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-July-2010 JSW
SynonymsProtein Tyrosine Phosphatase 1B
DescriptionRecombinant, human PTP1B expressed in E. coli. PTP1B, an ubiquitous nontransmembrane protein tyrosine phosphatase, was originally identified in human placenta. This highly active enzyme is useful for the study of tyrosine phosphatase kinetics, substrate specificity, and for screening inhibitors.
FormLiquid
FormulationIn 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.05% NP-40, pH 7.2.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥90% by SDS-PAGE
Specific activity≥20 units/mg
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphopeptide substrate (Cat. No. 539737) per min at 30°C, pH 7.2 using 150 µM of substrate.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesPuius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420.
Liu, F., et al. 1996. J. Biol. Chem. 271, 31290.