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14-602 PI3 Kinase (p110α/p85α) Protein, active, 20 µg

Active complex of N-terminal His6-tagged recombinant full-length human p110α & untagged, recombinant, full length, human p85α. For use in Kinase Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-602
20 µg  Also available in 250μg size as catalog # 14-602M - Call for pricing and availability.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-602
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPI3 Kinase (p110α/p85α) Protein, active, 20 µg
OverviewComplex of N-terminal His6-tagged recombinant full-length human p110α and untagged, recombinant, full length, human p85α
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive complex of N-terminal His6-tagged recombinant full-length human p110α & untagged, recombinant, full length, human p85α. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceCoexpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Gene Symbol
  • PIK3CA
  • PI3K
  • MGC142161
  • p110-alpha
  • MGC142163
Protein TargetPI3Kα/p85α
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyPI3K/PI4K
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Molecular Weightp110α (125.3kDa) & p85α (83.7kDa)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by PIProfiler
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size20 µg
Material PackageAlso available in 250μg size as catalog # 14-602M - Call for pricing and availability.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-602 04053252405501

Documentation

PI3 Kinase (p110α/p85α) Protein, active, 20 µg SDS

Title

Safety Data Sheet (SDS) 

PI3 Kinase (p110α/p85α) Protein, active, 20 µg Certificates of Analysis

TitleLot Number
PI3 Kinase (p110#945;/p85#945;), active - 1794230-F 1794230-F
PI3 Kinase (p110#945;/p85#945;), active - 1794230-M 1794230-M
PI3 Kinase (p110#945;/p85#945;), active - 1794230-N 1794230-N
PI3 Kinase (p110#945;/p85#945;), active - 1794230-O 1794230-O
PI3 Kinase (p110#945;/p85#945;), active - 1945160-A 1945160-A
PI3 Kinase (p110#945;/p85#945;), active - 2052238-A 2052238-A
PI3 Kinase (p110#945;/p85#945;), active - 2052238-C 2052238-C
PI3 Kinase (p110#945;/p85#945;), active - 2052238-D 2052238-D
PI3 Kinase (p110#945;/p85#945;), active - 2150294-A 2150294-A
PI3 Kinase (p110α/p85α), active - 2052238-E 2052238-E

Technical Info

Title
Unlocking the Mystery of Cancer: Do the Mutant p110α Subunits of PI3-Kinase Hold the Key?

Data Sheet

Title
PI 3-Kinase HTRF Assay

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-602M PI3 Kinase (p110α/p85α) Protein, active, 250 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases