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GF343 PDGF-AA,
Human Recombinant Animal Free

PDGF-AA,
Human Recombinant Animal Free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P04085
GF343
10µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SourcePurity
Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.		Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Description
Catalogue NumberGF343
DescriptionPDGF-AA,
Human Recombinant Animal Free
Alternate Names
  • PDGFA
  • PDGF-1
  • PDGF-A
  • PDGF1
Background InformationPDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR- α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGFBB and PDGF-AB.
Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
References
Product Information
PresentationProduct is presented in 10mM Acetic Acid and is filtered through a 0.2 micron filter before lyophilization.
Quality LevelMQ100
Applications
Biological Information
PurityProduct endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
SourceProtein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceDetermined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
Packaging Information
Material Size10µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF343 04053252979026

Documentation

PDGF-AA,
Human Recombinant Animal Free SDS

Title

Safety Data Sheet (SDS) 

PDGF-AA,
Human Recombinant Animal Free Certificates of Analysis

TitleLot Number
PDGF-AA, Human Recombinant Animal Free - Q2447016 Q2447016
PDGF-AA, Human Recombinant Animal Free - 3229429 3229429
PDGF-AA, Human Recombinant Animal Free - 3511587 3511587
PDGF-AA, Human Recombinant Animal Free - 3689441 3689441
PDGF-AA, Human Recombinant Animal Free - 3911798 3911798
PDGF-AA, Human Recombinant Animal Free - 4064291 4064291
PDGF-AA, Human Recombinant Animal Free Recombinant Protein 2853641
PDGF-AA,-2566794 2566794

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