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			96-Well Plate

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PCR Cleanup Filter Plates

Remove primers and unincorporated dNTPs in one step More

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Overview

Specifications

Ordering Information

Filter PlatesClear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pack Size
LSKMPCR10	MultiScreen PCR_µ96 Filter Plate	10
LSKMPCR50	MultiScreen-PCR_µ96 Filter Plate	50
MSNU03010	MultiScreen-PCR₉₆Filter Plate	10
MSNU03050	MultiScreen-PCR₉₆Filter Plate	50
S384PCR10	MultiScreen PCR₃₈₄Filter Plate	10
S384PCR50	MultiScreen PCR₃₈₄Filter Plate	50

Required EquimentClear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pack Size
MSVMHTS00	MultiScreen_HTS Vacuum Manifold	1
WP6111560	Chemical Duty Pump, 115 V/60 Hz	1
WP6122050	Chemical Duty Pump, 220 V/50 Hz	1
WP6110060	Chemical Duty Pump, 100 V/50–60 Hz	1

Documentation

References

Reference overview	Application
A Concise Guide to cDNA Microarray Analysis P. Hegde, R. Qi, K. Abernathy, C. Gay, S. Dharap, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee, and J. Quackenbush BioTechniques Vol. 29, No. 3: pp 548-562 (Sep 2000) 2000	PCR
PCR Amplification on a Microarray of Gel-Immobilized Oligonucleotides: Detection of Bacterial Toxin- and Drug-Resistant Genes and Their Mutations Strizhkov, B.N. Drobyshev, A.L. Mikhailovich, V.M. Mirzabekov, A.D. BioTechniques 29:844-857 2000
A specific and sensitive PCR assay suitable for large-scale detection of toxigenic Pasteurella multocida in nasal and tonsillar swabs specimens of pigs Kamp, E.M. etal., J.Vet Diagn Invest 8:, 304-309 J.Vet Diagn Invest 8:, 304-309 1996	Nucleic Acid Purification and Concentration
Use of MultiScreen Plates for the Preparation of Bacterial DNA Suitable for PCR Reek, F.H., Smits, M.A., Kamp, E. M., Smith, H.E. BioTechniques. 19: 2, 282 – 285 1995	Nucleic Acid Purification and Concentration

FAQ

Question	Answer
Can the Montage PCR-96 Kit be used in a procedure employing centrifugal rather than vacuum filtration?	Use of the Montage PCR-96 Kit in a centrifugal protocol is not recommended.
Can the MultiScreen plates included with the Montage kit be used with a vacuum manifold from a vendor other than Millipore?	Yes, but only if proper underdrain support grid is employed. Please contact your local Millipore technical service organization for specific information on the availability of the "underdrain supports" for non-Millipore vacuum manifolds.
Can the Montage PCRm96 plate be used in centrifugal mode?	No. The Montage PCRm96 plate is designed for purification using vacuum filtration only.
Which Millipore manifold do I use with the Montage PCRm96 plate?	The appropriate manifold is catalogue number SAVM38401. The unique micro-well design of the PCRm96 plate is not compatible with MultiScreen manifold catalogue number MAVM09601.
Will the Montage PCR96 and Montage PCRm96 plates remove primers and primer-dimers?	The Montage PCR96 Cleanup Kit is designed to remove primers and other low molecular weight material from the PCR reaction. However, removal of primer dimers is problematic. The Montage PCRm96 plate removes > 99% of PCR primers, but although small primer-dimers are efficiently removed, quantitative removal of larger primer-dimers can be problematic given that the PCRm96 plate has been optimized for recovery of small PCR products. The best way to avoid complications due to primer-dimers is to optimize primer design.
When using Montage PCRm96 plates, do I need to do anything special to increase the recovery of my 100 - 300 bp PCR fragments?	The protocol that was developed in conjunction with the PCRm96 plates has been optimized to provide maximal recovery of even small PCR products (e.g., 100 – 300 bp). No special modifications are necessary for small PCR fragments.
If I dilute my sample when using the Montage PCRu96 plate, will that increase the amount of time for filtration to be completed?	Yes. Although sample dilution will marginally increase processing time, dilution is essential for maximal recovery of PCR products.
Is a wash step required following the initial filtration when using the Montage PCRu96 plate?	The PCR product is sufficiently pure for DNA sequencing after a single vacuum filtration. If a particular application demands even higher purity, a wash step may be added.
What is the maximum vacuum that can be applied to the Montage PCRu96 plate?	The recommended vacuum pressure for PCR purification is 20 inches of Hg. Exceeding this vacuum pressure will adversely affect recovery of PCR products and is not recommended.
Is the Montage PCRm96 plate capable of removing Triton or other detergents from the PCR reaction?	No. The use of PCR reaction buffers that contain high concentrations of surfactants (i.e., greater than the critical micelle concentration) or protein stabilizers (e.g., gelatin) is not recommended for this application. Surfactants including Tween-20, Triton X-100 and Nonidet P-40 are not efficiently removed by the Montage PCRm96 plates and may result in carry-over into subsequent reactions.

Related Products & Applications

Categories

Life Science Research > Cell Culture and Systems > Cell Culture Flasks, Plates, & Slides > Multiwell Plates

C7479Remove primers and unincorporated dNTPs in one step

Our PCR filter plates are fast, automatable solutions for high-throughput PCR purification. Available in 96- and 384-well formats and a µ96-well format for small volumes, MultiScreen® PCR plates use a size-exclusion membrane and vacuum filtration to provide a one-step protocol with excellent results. No centrifugation or precipitation is required. Full sample recovery from the top of the plate enhances compatibility with liquid handling systems. MultiScreen® PCR_µ96 and MultiScreen®PCR₃₈₄ filter plates provide high recovery on even the smallest miniaturized reactions. Working volumes <150 µL conserve reagents and save money. For volumes >150 µL, use MultiScreen®PCR₉₆ 96-well filter plate.

Features & Benefits

High purity and high recovery
No centrifugation or precipitation
Fast processing times, automation compatible
MultiScreen® PCR_µ96 filter plate recommended for small fragments (1-150 bp)

Protocol

1.	Load PCR reactions.
2.	Filter with Millipore vacuum manifold for 5–10 minutes or until wells are dry.
3.	Add water or buffer to each well. Agitate by shaking or pipetting. Retrieve purified samples by aspiration.

Selection Guidelines

Specifications
Product name	Reaction volume	Number of samples	Mode of operation	Recommended applications	Features
MultiScreen^® PCR_µ96 Filter Plate	20 – 150 µL	96	Vacuum	Sequencing Genotyping Microarray	• Highly concentrated final sample • Reaction miniaturization
MultiScreen^® PCR₉₆ Filter Plate	150–300 µL	96	Vacuum	Microarray	• Volumetric capacity
MultiScreen^® PCR₃₈₄ Filter Plate	20–100 µL	384	Vacuum	Sequencing Genotyping Microarray	• Highly concentrated final sample • Reaction miniaturization • Ultra-high throughput

Recovery

Product name	Recovery of 137 bp PCR fragment*	Recovery of 301 bp PCR fragment*	Recovery of 657 bp PCR fragment*	Recovery of 1159 bp PCR fragment*	% Primer removal (20 bases), 100 µL volume
MultiScreen^® PCR_µ96 Filter Plate	+	++	++	++	99.8%
MultiScreen^® PCR₉₆ Filter Plate	--	+	++	++	98.7%
MultiScreen^® PCR₃₈₄ Filter Plate	+	++	++	++	99.5%
-- Not Recommended
+ Good Recovery
++ Best Recovery
*Results will vary based on starting concentration, load, and buffers used. Data obtained using the following concentrations: 137 bp: 10 ng/µL 301 bp: 30 ng/µL 657 bp: 55 ng/µL 1159 bp: 71 ng/µL

Performance

Get high reproducibility with MultiScreen PCR₉₆ Filter Plate

Agarose gel showing reproducibility of recovery of a 500 bp PCR product with MultiScreen PCR<sub>96</sub> filter plate.

Agarose gel showing reproducibility of recovery of a 500 bp PCR product with MultiScreen PCR₉₆ filter plate.

Long Sequencing Reads

Representative electropherogram from a 301 bp PCR product purified using MultiScreen PCR<sub>96</sub> filter plate (Thermo Sequenase® II dye terminator).

Representative electropherogram from a 301 bp PCR product purified using MultiScreen PCR₉₆ filter plate (Thermo Sequenase® II dye terminator).

Suitable for Microarrays

Enlarged section of a 19,299 element microarray (image reproduced with permission from Dr. John Quakenbush, The Institute for Genomic Research). High quality microarrays can be manufactured using PCR products purified with MultiScreen PCR products. Reference: Hegde, P., R. Qi, K. Abernathy, C. Gay, S. Dhaarp, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee and J. Quakenbush. 2000. A concise guide to cDNA microarray analysis. BioTechniques 29: 548–562.

Applications

PCR Cleanup, Sequencing, Genotyping, Microarray, RNAi Purification, Microarray Purification, Genomic DNA Concentration

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