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FCMAB230P Milli-Mark™ Mouse IgG1-k, clone MOPC-21, PE conjugate

FCMAB230P
100 tests  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMPhycoerythrinMonoclonal Antibody
Description
Catalogue NumberFCMAB230P
Trade Name
  • Milli-Mark
DescriptionMilli-Mark™ Mouse IgG1-k, clone MOPC-21, PE conjugate
Background InformationThe MOPC-21 tumor line that produces mouse IgG1,k, is a mineral oil induced plasmacytoma. The product is originated and carried intraperitoneally in BALB/c mice. Identity and purity of the immunoglobulin is established by immunoelectrophoresis (IEP) and Ouchterlony Double Diffusion (ODD), prior to conjugation. Electrophoresis of the purified immunoglobulin, followed by diffusion versus anti-mouse whole serum, antimouse IgG1 and anti-mouse kappa chain, results in single arcs of precipitation. By ODD the purified immunoglobulin preparation is non-reactive with antisera to mouse lambda light chain, mouse IgA, IgM, IgG2a, IgG2b, and IgG3. The specificity of staining by monoclonal antibodies to human CD antigens should be verified by establishing the amount of non-specific binding to the target cell population. It is recommended that a non-reactive immunoglobulin of the same isotype, concentration and F/P molar ratio be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
References
Product Information
FormatPhycoerythrin
PresentationPurified mouse monoclonal IgG1κ conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationThis Milli-Mark Anti-mouse IgG1-PE Antibody, clone MOPC-21 is validated for use in FC for the detection of mouse IgG1.
Key Applications
  • Flow Cytometry
Biological Information
CloneMOPC-21
HostMouse
SpecificityThis mouse IgG1 isotype control antibody is not reactive with any known antigen.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Purification MethodImmunoelectrophoresis
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated for use as flow cytometry isotype control using Jurkat Cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCMAB230P 04053252680908

Documentation

Milli-Mark™ Mouse IgG1-k, clone MOPC-21, PE conjugate SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark™ Mouse IgG1-k, clone MOPC-21, PE conjugate Certificates of Analysis

TitleLot Number
Milli-Mark Anti-mouse IgG1-PE, clone MOPC-21 - 1959255 1959255
Milli-Mark Anti-mouse IgG1-PE, clone MOPC-21 - 1977620 1977620
Milli-Mark Anti-mouse IgG1-PE, clone MOPC-21 - 1981634 1981634
Milli-Mark Anti-mouse IgG1-PE, clone MOPC-21 - 2003684 2003684
Milli-Mark Anti-mouse IgG1-PE, clone MOPC-21 - NRG1813747 NRG1813747
Milli-Mark Mouse IgG1-k, clone MOPC-21, PE conjugate - 2105229 2105229
Milli-Mark Mouse IgG1-k, clone MOPC-21, PE conjugate - 2227204 2227204
Milli-Mark™ Mouse IgG1-k, clone MOPC-21, PE conjugate 2961171
Milli-Mark™ Mouse IgG1-k, clone MOPC-21, PE conjugate 3089185
Milli-Mark™ Mouse IgG1-k, clone MOPC-21, PE conjugate 2922303