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14-524M MST2 Protein, active, 250 µg

Active, N-terminal His6-tagged recombinant human MST2 residues 2-end, for use in Kinase Assays.

MST2 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-524M
250 µg  Also available in 10 μg size (cat#14-524) and in bulk (cat# 14-524-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-524M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMST2 Protein, active, 250 µg
OverviewN-terminal His6-tagged recombinant human MST2 residues 2-end
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged recombinant human MST2 residues 2-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryProtein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]
Gene Symbol
  • STK3
  • MST-2
  • FLJ90748
  • MST2
  • KRS1
Protein TargetMST2
Purification MethodNi2+/NTA-agarose
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q13188 # Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Phosphorylates TITF1 (By similarity). Phosphorylates and activates LATS1 and LATS2.
COFACTOR: Magnesium.
SIZE: 491 amino acids; 56301 Da
SUBUNIT: Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (By similarity). Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1, which leads to enzyme activation.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=The caspase-cleaved form cycles between nucleus and cytoplasm (By similarity).
TISSUE SPECIFICITY: Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
SIMILARITY: SwissProt: Q13188 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 protein kinase domain. & Contains 1 SARAH domain.
Molecular Weight57.2kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of MBP
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-524) and in bulk (cat# 14-524-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-524M 04053252511820

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-524 MST2 Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases