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14-377 MKK4/SKK1 Protein, active, 10 µg

Active, N-terminal GST tagged, murine MKK4 residues 34-end, for use in Kinase Assays.

MKK4/SKK1 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-377
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-377M when ordering the 250 µg size.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-377
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMKK4/SKK1 Protein, active, 10 µg
OverviewN-terminal GST tagged, murine MKK4 residues 34-end
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal GST tagged, murine MKK4 residues 34-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in E. coli
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
Gene Symbol
  • MAP2K4
  • MKK4
  • JNKK
  • MEK4
  • MAPKK4
  • JNKK1
  • SEK1
  • SERK1
  • PRKMK4
Protein TargetMKK4
Purification MethodPurified using glutathione agarose. Activated by incubation with MEKK and then re-purified using Ni2+/NTA agarose
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P45985 # Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
SIZE: 399 amino acids; 44288 Da
SUBUNIT: Interacts with SPAG9 (By similarity).
TISSUE SPECIFICITY: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
PTM: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases (By similarity).
SIMILARITY: SwissProt: P45985 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight67.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of ATF2 substrate
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-377M when ordering the 250 µg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-377 04053252673337

Documentation

MKK4/SKK1 Protein, active, 10 µg SDS

Title

Safety Data Sheet (SDS) 

MKK4/SKK1 Protein, active, 10 µg Certificates of Analysis

TitleLot Number
MKK4/SKK1, active - D8JN050U-M D8JN050U-M
MKK4/SKK1, active - D8JN050U-N D8JN050U-N
MKK4/SKK1, active - D8JN050U-O D8JN050U-O
MKK4/SKK1, active - D8JN050U-P D8JN050U-P
MKK4/SKK1, active - D8JN050U-Q D8JN050U-Q

References

Reference overviewApplicationPub Med ID
Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7
Fleming, Y., et al
Biochem J, 352 Pt 1:145-54 (2000)  2000

Kinase Assay11062067 11062067

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-377M MKK4/SKK1 Protein, active, 250 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases