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429700 Leptin, Human, Recombinant, E. coli

Leptin, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhOB

429700
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
429700-1MG Plastic ampoule 1 mg
Description
OverviewRecombinant, human leptin expressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations of the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism, and body temperature. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis. Note: Following complete dissolution in 15 mM HCl, add 7.5 mM sterile NaOH and bring the pH to approximately 5.2.
Catalogue Number429700
Brand Family Calbiochem®
SynonymsrhOB
References
ReferencesAnderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
Ookuma, M., et al. 1998. Diabetes 47, 219.
Campfield, L.A., et al. 1995. Science 269, 546.
Halaas, J.L., et al. 1995. Science 269, 543.
Pelleymounter, M.A., et al. 1995. Science 269, 540.
Zhang, Y., et al. 1994. Nature 372, 425.
Product Information
CAS number177404-21-6
FormLyophilized
FormulationLyophilized from a sterile filtered solution in PBS.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 0.4-2 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg leptin
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsTo reconstitute lyophilized leptin, add 15 mM sterile HCl (0.5 ml/1 mg vial or 2.5 ml/5 mg vial) to the vial. After the protein is completely dissolved, add 7.5 mM sterile NaOH (0.3 ml/1mg vial or 1.5 ml/5 mg vial) to bring the pH to ~5.2. Lyophilized samples are stable for at least six months at -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 4°C for one month or at -70°C for three months without detectable loss of activity. Avoid repeated freeze/thaw cycles of reconstituted solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
429700-1MG 04055977210132

Documentation

Leptin, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Leptin, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
429700

References

Reference overview
Anderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
Ookuma, M., et al. 1998. Diabetes 47, 219.
Campfield, L.A., et al. 1995. Science 269, 546.
Halaas, J.L., et al. 1995. Science 269, 543.
Pelleymounter, M.A., et al. 1995. Science 269, 540.
Zhang, Y., et al. 1994. Nature 372, 425.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-May-2008 RFH
SynonymsrhOB
DescriptionRecombinant, human leptin expressed in E. coli. Leptin was originally identified as a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. Human leptin shares approximately 84% sequence identity with the mouse protein. Human leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid signal sequence that is cleaved to yield the 146 amino acid mature protein. The expression of leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for leptin (OB-R) with homology to gp130 and the G-CSF receptor was subsequently cloned. The OB-R cytoplasmic domain transduces the leptin signal through the JAK-STAT pathway. Although OB-R mRNA was initially shown to be expressed predominantly in the choroid plexus and in the hypothalamus, more recent data also revealed the expression of this receptor in endothelial cells (Ecs). Furthermore, the angiogenic activity of leptin has been demonstrated both in vitro and in vivo, suggesting a physical mechanism whereby leptin-induced angiogenesis may facilitate increased energy expenditure.
FormLyophilized
FormulationLyophilized from a sterile filtered solution in PBS.
CAS number177404-21-6
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg leptin
Biological activityED₅₀ = 0.4-2 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsTo reconstitute lyophilized leptin, add 15 mM sterile HCl (0.5 ml/1 mg vial or 2.5 ml/5 mg vial) to the vial. After the protein is completely dissolved, add 7.5 mM sterile NaOH (0.3 ml/1mg vial or 1.5 ml/5 mg vial) to bring the pH to ~5.2. Lyophilized samples are stable for at least six months at -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 4°C for one month or at -70°C for three months without detectable loss of activity. Avoid repeated freeze/thaw cycles of reconstituted solutions.
Toxicity Standard Handling
Merck USA index14, 5443
ReferencesAnderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
Ookuma, M., et al. 1998. Diabetes 47, 219.
Campfield, L.A., et al. 1995. Science 269, 546.
Halaas, J.L., et al. 1995. Science 269, 543.
Pelleymounter, M.A., et al. 1995. Science 269, 540.
Zhang, Y., et al. 1994. Nature 372, 425.