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420119 JNK Inhibitor II - CAS 129-56-6 - Calbiochem

JNK Inhibitor II, CAS 129-56-6, is a cell-permeable, potent, selective, ATP-competitive, and reversible inhibitor of c-Jun N-terminal kinase (IC50 = 40 nM for JNK-1 & 2 & 90 nM for JNK-3).

JNK Inhibitor II - CAS 129-56-6 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anthra[1,9-cd]pyrazol-6(2H)-one, 1,9-pyrazoloanthrone, SP600125, SAPK Inhibitor II

Primary Target: JNK 1, JNK 2, JNK 3
420119
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
129-56-6C₁₄H₈N₂O

Products

Catalogue NumberPackaging Qty/Pack
420119-25MG Plastic ampoule 25 mg
420119-50MG Plastic ampoule 50 mg
420119-5MG Plastic ampoule 5 mg
Description
OverviewA potent, cell-permeable, selective, and reversible inhibitor of c-Jun N-terminal kinase (JNK) (IC50 = 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation of c-Jun and blocks cellular expression of IL-2, IFN-γ, TNF-α, and COX-2. Blocks IL-1-induced accumulation of phospho-Jun and induction of c-Jun transcription. A 50 mM (5 mg/454 µl) solution of JNK Inhibitor II (Cat. No. 420128) in DMSO is also available.
Catalogue Number420119
Brand Family Calbiochem®
SynonymsAnthra[1,9-cd]pyrazol-6(2H)-one, 1,9-pyrazoloanthrone, SP600125, SAPK Inhibitor II
References
ReferencesShin, M., and Boyd, Y.D. 2002. Biochim. Biophys. Acta 1589, 311.
Bennett, B.L., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 13681.
Han, Z., et al. 2001. J. Clin. Invest. 108, 73.
Product Information
CAS number129-56-6
ATP CompetitiveY
FormYellowish orange solid
Hill FormulaC₁₄H₈N₂O
Chemical formulaC₁₄H₈N₂O
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ200
Applications
ApplicationJNK Inhibitor II, CAS 129-56-6, is a cell-permeable, potent, selective, ATP-competitive, and reversible inhibitor of c-Jun N-terminal kinase (IC50 = 40 nM for JNK-1 & 2 & 90 nM for JNK-3).
Biological Information
Primary TargetJNK 1, JNK 2, JNK 3
Primary Target IC<sub>50</sub>40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSCB4585000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
420119-25MG 04055977187977
420119-50MG 04055977187991
420119-5MG 07790788050092

Documentation

JNK Inhibitor II - CAS 129-56-6 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

JNK Inhibitor II - CAS 129-56-6 - Calbiochem Certificates of Analysis

TitleLot Number
420119

References

Reference overview
Shin, M., and Boyd, Y.D. 2002. Biochim. Biophys. Acta 1589, 311.
Bennett, B.L., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 13681.
Han, Z., et al. 2001. J. Clin. Invest. 108, 73.

Posters

Title
Human Kinome & InhibitorSelect™ Libraries

Citations

Title
  • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
  • Yuseok Moon, Wayne C. Glasgow and Thomas E. Eling. (2005) Curcumin suppresses interleukin 1β-mediated microsomal prostaglandin E synthase 1 (mPGES-1) by altering early growth response gene EGR-1 and other signaling pathways. Journal of Pharmacology and Experimental Therapeutics in press,.
  • Naoto Inukai, et al. (2004) A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. Journal of Biological Chemistry 279, 8190-8195.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision08-June-2009 RFH
    SynonymsAnthra[1,9-cd]pyrazol-6(2H)-one, 1,9-pyrazoloanthrone, SP600125, SAPK Inhibitor II
    DescriptionA potent, cell-permeable, selective, and reversible inhibitor of c-Jun N-terminal kinase (JNK) (IC50 = 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation of c-Jun and blocks the expression of IL-2, IFN-γ, TNF-α, and COX-2 in cells. Blocks IL-1-induced accumulation of phospho-Jun and induction of c-Jun transcription.
    FormYellowish orange solid
    Intert gas (Yes/No) Packaged under inert gas
    CAS number129-56-6
    RTECSCB4585000
    Chemical formulaC₁₄H₈N₂O
    Structure formulaStructure formula
    Purity≥98% by HPLC
    SolubilityDMSO (15 mg/ml). For every 10 µM concentration of JNK Inhibitor II, include 0.1% DMSO in the culture medium. Pre-warming of culture medium and addition of BSA to the medium may enhance its solubility.
    Storage Protect from light
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.
    Toxicity Standard Handling
    ReferencesShin, M., and Boyd, Y.D. 2002. Biochim. Biophys. Acta 1589, 311.
    Bennett, B.L., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 13681.
    Han, Z., et al. 2001. J. Clin. Invest. 108, 73.
    Citation
  • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
  • Yuseok Moon, Wayne C. Glasgow and Thomas E. Eling. (2005) Curcumin suppresses interleukin 1β-mediated microsomal prostaglandin E synthase 1 (mPGES-1) by altering early growth response gene EGR-1 and other signaling pathways. Journal of Pharmacology and Experimental Therapeutics in press,.
  • Naoto Inukai, et al. (2004) A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. Journal of Biological Chemistry 279, 8190-8195.
    		•