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344092 Formin FH2 Domain Inhibitor, SMIFH2 - CAS 340316-62-3 - Calbiochem

Formin FH2 Domain Inhibitor, SMIFH2, CAS 340316-62-3, is a cell-permeable inhibitor of formin-mediated actin nucleation (IC50 ~15 µM) and formin-mediated elongation of actin filaments.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Small Molecule Inhibitor of Formin Homology 2 domain, 1-(3-Bromophenyl)-5-(2-furylmethylene)-2-thioxo-hexahydropyrimidine-4,6-dione, Actin Assembly Inhibitor XV

344092
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
340316-62-3C₁₅H₉BrN₂O₃S

Products

Catalogue NumberPackaging Qty/Pack
344092-10MG Glass bottle 10 mg
Description
OverviewA cell-permeable thiooxodihydropyrimidinedione compound that inhibits both formin-mediated, profilin-independent actin nucleation (IC50 ~15 µM using mDia1 or mDia2) and formin-mediated elongation of actin filaments in the presence of profilin (IC50 ~4 µM using Cdc12 or mDia2), but not the Arp2/3-mediated or formin-independent actin assembly. SMIFH2 targets the FH2 (formin homology 2) domain of formins from a large variety of species, including murine mDia1/2, C. elegans CYK-1, S. pombe Cdc12, S. pombe Fus1, and S. cerevisiae Bni1, and decreases formin affinity for the actin filament barbed end. SMIFH2, at 25 µM, is shown to selectively disrupt formin-dependent actin cables and contractile rings, but not Arp2/3-dependent, CK-666- (Cat. No. 182515) sensitive actin patches, in fission yeast. SMIFH2 is also demonstrated to affect F-actin cytoskeleton structures and cell migration (by a 2-fold decrease at 10 µM) in NIH 3T3 fibroblast cultures.
Catalogue Number344092
Brand Family Calbiochem®
SynonymsSmall Molecule Inhibitor of Formin Homology 2 domain, 1-(3-Bromophenyl)-5-(2-furylmethylene)-2-thioxo-hexahydropyrimidine-4,6-dione, Actin Assembly Inhibitor XV
References
ReferencesRizvi, S.A., et al. 2009. Chem. Biol. 16, 1158.
Product Information
CAS number340316-62-3
FormDark to deep green solid
Hill FormulaC₁₅H₉BrN₂O₃S
Chemical formulaC₁₅H₉BrN₂O₃S
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationFormin FH2 Domain Inhibitor, SMIFH2, CAS 340316-62-3, is a cell-permeable inhibitor of formin-mediated actin nucleation (IC50 ~15 µM) and formin-mediated elongation of actin filaments.
Biological Information
Purity≥98% by HPLC (sum of two geometrical isomers)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsUse only fresh DMSO. Following reconstiution, aliquot an freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
344092-10MG 04055977194579

Documentation

Formin FH2 Domain Inhibitor, SMIFH2 - CAS 340316-62-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Formin FH2 Domain Inhibitor, SMIFH2 - CAS 340316-62-3 - Calbiochem Certificates of Analysis

TitleLot Number
344092

References

Reference overview
Rizvi, S.A., et al. 2009. Chem. Biol. 16, 1158.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-August-2016 JSW
SynonymsSmall Molecule Inhibitor of Formin Homology 2 domain, 1-(3-Bromophenyl)-5-(2-furylmethylene)-2-thioxo-hexahydropyrimidine-4,6-dione, Actin Assembly Inhibitor XV
DescriptionA cell-permeable thiooxodihydropyrimidinedione compound that inhibits both formin-mediated, profilin-independent actin nucleation (IC50 ~15 µM using mDia1 or mDia2) and formin-mediated elongation of actin filaments in the presence of profilin (IC50 ~4 µM using Cdc12 or mDia2), but not the Arp2/3-mediated or formin-independent actin assembly. SMIFH2 targets the FH2 (formin homology 2) domain of formins from a large variety of species, including murine mDia1/2, C. elegans CYK-1, S. pombe Cdc12, S. pombe Fus1, and S. cerevisiae Bni1, and decreases formin affinity for the actin filament barbed end. SMIFH2, at 25 µM, is shown to selectively disrupt formin-dependent actin cables and contractile rings, but not Arp2/3-dependent, CK-666- (Cat. No. 182515) sensitive actin patches, in fission yeast. SMIFH2 is also demonstrated to affect F-actin cytoskeleton structures and cell migration (by a 2-fold decrease at 10 µM) in NIH 3T3 fibroblast cultures.
FormDark to deep green solid
Intert gas (Yes/No) Packaged under inert gas
CAS number340316-62-3
Chemical formulaC₁₅H₉BrN₂O₃S
Structure formulaStructure formula
Purity≥98% by HPLC (sum of two geometrical isomers)
SolubilityDMSO (50 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsUse only fresh DMSO. Following reconstiution, aliquot an freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesRizvi, S.A., et al. 2009. Chem. Biol. 16, 1158.