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HTS138M ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor

Human CXCR7 / RDC1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Uni Prot Number: P25106 Entrez Gene Number: NM_020311.2
HTS138M
200 units  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-1CXCR7 / RDC1
Description
Catalogue NumberHTS138M
Trade Name
  • ChemiScreen
  • Chemicon
DescriptionChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor
Overview: Full-length human CXCR7 cDNA encoding CXCR7
Background InformationThe orphan receptor RDC1 is phylogenetically related to chemokine receptors, and its gene (CMKOR1, chemokine orphan receptor 1) occupies a chromosomal location near the genes for other CXC receptors. Several immune system cells, including T lymphocytes, mature dendritic cells and memory B cells, express RDC1 (Infantino et al., 2006). A subset of HIV and SIV strains use RDC1 as a coreceptor (Shimizu et al., 2000). The chemokine SDF-1/CXCL12, which binds to CXCR4, has been shown also to bind and activate RDC1 to promote chemotaxis of T lymphocytes; thus, CXCR7 has been proposed to be the new name for RDC1 (Balabanian et al., 2005). I-TAC, one of the chemokines thought to bind to CXCR3 only, has been reported to distinguish CXCR7 from CXCR4 in radioligand binding experiments (Burns et al. 2006). Chemicon's CXCR7 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at CXCR7. The membrane preparations exhibit a Kd of 0.32 nM for [125I]-SDF-1α. With 0.25 nM [125I]-SDF-1α, 10µg/well CXCR7 Membrane Prep typically yields greater than 4-fold signal-to-background ratio.
References
Product Information
FormatMembranes
PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to 1 mg/mL in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80°C.
Quality LevelMQ100
Applications
ApplicationHuman CXCR7 / RDC1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Key Applications
  • Radioligand binding assay
  • GTPγS binding
Application NotesRadioligand binding assay and GTPγS binding
Biological Information
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas.
Gene Symbol
  • CXCR7
  • CXCR-7
  • GPR159
  • CMKOR1
  • RDC-1
  • CXC-R7
  • RDC1
GPCR ClassA
Host CellsChem-1
Protein TargetCXCR7 / RDC1
Target Sub-FamilyChemokine
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P25106 # Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
SIZE: 362 amino acids; 41493 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in monocytes, basophils, and B- cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
SIMILARITY: SwissProt: P25106 ## Belongs to the G-protein coupled receptor 1 family.
Incubation ConditionsMembranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] SDF-1α (Perkin Elmer#: NEX-346)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.

One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 4-fold signal:background with 125I-labeled SDF-1α at 0.25 nM
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Table 1. Signal:background and specific binding values obtained in a competition binding assay with CXCR7 membrane prep and unlabeled SDF-1α.
Product Usage Statements
10 µg/well
Signal:Background 4.92
Specific Binding (cpm) 15919

1 unit = 10 µg
Bmax for [125I]- SDF-1α binding: 0.51 pmol/mg protein
Kd for [125I]- SDF-1α binding: ~0.32 nM Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Packaging Information
Material Size200 units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS138M 04053252626173

Documentation

ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor SDS

Title

Safety Data Sheet (SDS) 

ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor Certificates of Analysis

TitleLot Number
CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN CXCR7 CHEMOKINE RECEPTOR - 2051433 2051433
CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN CXCR7 CHEMOKINE RECEPTOR - 2144905 2144905

References

Reference overviewPub Med ID
A novel chemokine receptor for SDF-1 and I-TAC involved in cell survival, cell adhesion, and tumor development
Burns, Jennifer M, et al
J Exp Med, 203:2201-13 (2006)  2006

16940167 16940167

Data Sheet

Title
CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN CXCR7 CHEMOKINE RECEPTOR

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