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14-731 CaM Kinase Iδ Protein, active, 20 µg

Active, N-terminal 6His-tagged, recombinant, human, full length CaM Kinase Iδ. For use in Enzyme Assays.

CaM Kinase Iδ Protein, active, 20 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-731
20 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-731
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCaM Kinase Iδ Protein, active, 20 µg
OverviewN-terminal 6His-tagged, recombinant, human, full length CaM Kinase Iδ.
Alternate Names
  • Calcium/calmodulin-dependent protein kinase type 1D
  • CaMKI-delta
References
Product Information
PresentationPresented as a frozen liquid in 50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human, full length CaM Kinase Iδ. For use in Enzyme Assays.
Key Applications
  • Enzyme Assays
Biological Information
ConcentrationFor concentration, please refer to the Certificate of Analysis for individual lots of this enzyme.
PurityFor purity information, please refer to the Certificate of Analysis for individual lots of this enzyme.
SourceRecombinant human protein expressed by baculovirus in Sf21 cells.
Specific ActivityFor Specific Activity data, please refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the Ca2+/calmodulin-dependent protein kinase 1 subfamily of serine/threonine kinases. The encoded protein may be involved in the regulation of granulocyte function through the chemokine signal transduction pathway. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Gene Symbol
  • CAMK1D
  • CaM-K1
  • CaMKI-delta
  • Camk1D
  • CKLiK
  • OTTHUMP00000045030
  • CaMKID
Protein TargetCaMKIδ
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q8IU85 # Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. Activates MAP kinase MAPK3 (By similarity). In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1.
SIZE: 385 amino acids; 42914 Da
SUBCELLULAR LOCATION: Cytoplasm (Probable). Nucleus (Probable). Note=Predominantly cytoplasmic (Probable). Also nuclear upon activation (Probable).
TISSUE SPECIFICITY: Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes.
DOMAIN:SwissProt: Q8IU85 The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.
Molecular Weight46.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 150μM KKLRRTLSVA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -70°C from date of shipment. Upon first use, rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70deg;C.
Packaging Information
Material Size20 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-731 04053252584725

Documentation

CaM Kinase Iδ Protein, active, 20 µg SDS

Title

Safety Data Sheet (SDS) 

CaM Kinase Iδ Protein, active, 20 µg Certificates of Analysis

TitleLot Number
CaM Kinase Id, active - 31350U-A 31350U-A

FAQ

QuestionAnswer
What is the Molar extinction coefficient for Adenosine 5'-[g32P]triphosphate ([g32P]ATP) used in the kinase assay?The Molar extinction coefficient is 15400.
What is the best way to store and thaw enzymes?In order to maintain activity of enzymes, we recommend a slow thaw and aliquot storage. To do this, remove the full vial from the freezer and place under cold running tap water. Once thawed, place the vial on ice, gently mix, than make aliquots. Using liquid nitrogen or an ethanol/dry ice bath, flash freeze the aliquots. Store aliquots at the recommended temperature.

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Alternative Packsize

Catalogue Number Description
14-731M CaM Kinase Iδ Protein, active, 250 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases