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71206 Benzonase® Nuclease HC, Purity > 99%

Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71206
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
71206-3 Glass bottle 25 ku
Description
OverviewBenzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination. The ability of Benzonase to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein. For example, the enzyme is compatible with BugBuster® and PopCulture® Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts.

The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mM ammonium sulfate, or > 100 mM guanidine HCl.

Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/µl and at a high concentration (HC) of 250 U/µl. Both preparations are free of detectable protease and have specific activity > 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains < 0.25 EU/1000 units. The product is supplied as a 0.2 µm filtered solution in 50% glycerol. Store at -20°C.

Total endotoxin: < 0.25 EU/1,000 units. Purity: > 99% by SDS-PAGE.
Catalogue Number71206
Brand Family Novagen®
References
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
Quality LevelMQ100
Applications
Biological Information
Purity> 99% by SDS-PAGE.
Physicochemical Information
ContaminantsTotal endotoxin: < 0.25 EU/1000 units.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71206-3 04055977271058

Documentation

Benzonase® Nuclease HC, Purity > 99% Certificates of Analysis

TitleLot Number
71206

Brochure

Title
Bulk Product Guide
Overnight Express Autoinduction Systems
Protein Purification and Detection Tools

Technical Info

Title
Benzonase®endonuclease for improved primary recovery in an E. coli-based process for Fab production

Citations

Title
  • Lisa Kercher, et al. (2007) Prion protein (PrP) expression differences in microglia and astroglia influence scrapie-induced neurodegeneration in retina and brain of transgenic mice. Journal of Virology 81, 10340-10351.
  • Shogo Kobayashi, et al. (2007) Serine 64 phosphorylation enhances the antiapoptotic function of Mcl-1. Journal of Biological Chemistry 282, 18407-18417.
  • Kentaro Miyazaki, et al. (2006) Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution. Journal of Biological Chemistry 281, 10236-10242.
  • Erumbi S. Rangarajan, et al. (2005) Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases. Journal of Biological Chemistry 280, 42919-42928.
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    User Protocols

    Title
    TB261 Benzonase® Nuclease