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05-368MG Anti-phospho-Ser/Thr-Pro MPM-2 Antibody

05-368MG
1 mg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtELISA, IHC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-368MG
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Ser/Thr-Pro MPM-2 Antibody
OverviewThis antibody recognizes a variety of proteins that are phosphorylated during mitosis.
Background InformationUpon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.
References
Product Information
FormatPurified
Control
  • Colcemid-treated HeLa (lane 2) cell lysates, A431 mitotic cells fixed with 3.7% paraformaldehyde
PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in mouse IgG in 1.0 mL PBS containing no preservatives.
Quality LevelMQ100
Applications
ApplicationDetect phospho-Ser/Thr-Pro MPM-2 also known as Mitotic protein #2 with Anti-phospho-Ser/Thr-Pro MPM-2 Antibody (Mouse Monoclonal Antibody), that has been demonstrated to work in ELISA, IHC, IP & WB.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Biological Information
ImmunogenMitotic human HeLa cell cytosolic lysate
CloneMPM-2
HostMouse
SpecificityRecognizes phosphorylated serines or threonines when followed by a proline.
IsotypeIgG1
Species Reactivity
  • Vertebrates
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G Purified
Molecular Weightvaries depending on the protein detected
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-368MG 04053252507151

Documentation

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-Ser/Thr-Pro, MPM-2 (mouse monoclonal IgG1) - 2117728 2117728
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 0702051572 0702051572
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 0704056977 0704056977
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 27704 27704
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 31404 31404
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - DAM1408764 DAM1408764
Anti-phospho-Ser/Thr-Pro, MPM-2 (mouse monoclonal IgG1) 2970639
Anti-phospho-Ser/Thr-Pro, MPM-2 (mouse monoclonal IgG1) 3115881
Anti-phospho-Ser/Thr-Pro, MPM-2 (mouse monoclonal IgG1) - 2328345 2328345
Anti-phospho-Ser/Thr-Pro, MPM-2 - 2424745 2424745

References

Reference overviewApplicationPub Med ID
Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation.
Whalley, HJ; Porter, AP; Diamantopoulou, Z; White, GR; Castañeda-Saucedo, E; Malliri, A
Nature communications  6  7437  2015

Show Abstract
26078008 26078008
Prdx4 is a compartment-specific H2O2 sensor that regulates neurogenesis by controlling surface expression of GDE2.
Yan, Y; Wladyka, C; Fujii, J; Sockanathan, S
Nature communications  6  7006  2015

Show Abstract
25943695 25943695
Sequence-specific and phosphorylation-dependent proline isomerization: a potential mitotic regulatory mechanism.
Yaffe, M B, et al.
Science, 278: 1957-60 (1997)  1997

Show Abstract
Immunoblotting (Western)9395400 9395400
Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope.
Westendorf, J M, et al.
Proc. Natl. Acad. Sci. U.S.A., 91: 714-8 (1994)  1994

Show Abstract
8290587 8290587
The mitosis-specific monoclonal antibody MPM-2 recognizes phosphoproteins associated with the nuclear envelope in Chlamydomonas reinhardtii cells.
Harper, J D, et al.
Eur. J. Cell Biol., 51: 272-8 (1990)  1990

Show Abstract
1693575 1693575
Mitosis-specific monoclonal antibody MPM-2 inhibits Xenopus oocyte maturation and depletes maturation-promoting activity.
Kuang, J, et al.
Proc. Natl. Acad. Sci. U.S.A., 86: 4982-6 (1989)  1989

Show Abstract
2662192 2662192
Phosphoproteins are components of mitotic microtubule organizing centers.
Vandre, D D, et al.
Proc. Natl. Acad. Sci. U.S.A., 81: 4439-43 (1984)  1984

Show Abstract
6379644 6379644
Monoclonal antibodies to mitotic cells.
Davis, F M, et al.
Proc. Natl. Acad. Sci. U.S.A., 80: 2926-30 (1983)  1983

Show Abstract
6574461 6574461

Related Products & Applications

Alternative Packsize

Catalogue Number Description
05-368 Anti-phospho-Ser/Thr-Pro MPM-2 Antibody
05-368-KC Anti-phospho-Ser/Thr-Pro, MPM-2 (KC)

Alternative Format

Catalogue Number Description
16-155 Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, FITC conjugate
16-220 Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, Cy5 conjugate

Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies