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07-896-I Anti-phospho-Rac1/cdc42 (Ser71) Antibody

Detect Rac1/CDC42 using this rabbit Polyclonal Anti-phospho-Rac1/cdc42 (Ser71) Antibody, Cat. No. 07-896-I, validated for use in Western Blotting, Immunoprecipitation.

Anti-phospho-Rac1/cdc42 (Ser71) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: RAC1, TC25, MIG5 UniProt Number: P63000
07-896-I
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-896-I
DescriptionAnti-phospho-Rac1/cdc42 (Ser71) Antibody
Alternate Names
  • Ras-related C3 botulinum toxin substrate 1
  • Cell migration-inducing gene 5 protein
  • Ras-like protein TC25
  • p21-Rac1
  • phospho-Rac1/cdc42 (Ser71)
Background InformationTarget of rapamycin complex 2 subunit MAPKAP1 (UniProt Q9BPZ7; also known as MEKK2-interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1, mSIN1, Ras inhibitor MGC2745, SAPK-interacting protein 1, Stress-activated protein kinase-interacting 1, Stress-activated map kinase-interacting protein 1,, TORC2 subunit MAPKAP1) is encoded by the MAPKAP1 (also known as JC310, MIP1 SIN1, SIN1b, SIN1g) gene (Gene ID 79109) in human. mSIN1 is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase, along with mSIN1, GbL (mLST8), Protor1, Protor2, and Rictor. The mTORC2 complex is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is known to affect cytoskeletal organization and cell migration by exerting its effects through Rac, Rho, and PKC. mSIN1 is also known to directly interact with Ras, SPAKs, and JNK.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Rac1/CDC42 using this rabbit Polyclonal Anti-phospho-Rac1/cdc42 (Ser71) Antibody, Cat. No. 07-896-I, validated for use in Western Blotting, Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: 2.5µg from a representative lot immunoprecipitated phospho-Rac1/cdc42 (Ser71) in EGF treated A431 cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human phospho-Rac1/cdc42 (Ser71) near the N-Terminus.
EpitopeNear N-Terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Rhesus Macaque, Xenopus, Zebrafish, Chicken , Orangatan based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAC1
  • TC25
  • MIG5
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~25 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in EGF treated A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Rac1/cdc42 (Ser71) in 10 µg of EGF treated A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-896-I 04055977176438

Documentation

Anti-phospho-Rac1/cdc42 (Ser71) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Rac1/cdc42 (Ser71) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-Rac1/cdc42 (Ser71) - 3389918 3389918
Anti-phospho-Rac1/cdc42 (Ser71) - 3934291 3934291
Anti-phospho-Rac1/cdc42 (Ser71) - 4082547 4082547
Anti-phospho-Rac1/cdc42 (Ser71) -Q2581246 Q2581246

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.