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ABS403 Anti-phospho NFkB p65 Antibody, (Ser547)

Anti-phospho NFkB p65 Antibody, (Ser547) is an antibody against phospho NFkB p65 Antibody for use in Western Blotting.

Anti-phospho NFkB p65 Antibody, (Ser547) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: RELA, NFKB3 UniProt Number: Q04206
ABS403
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS403
DescriptionAnti-phospho NFkB p65 Antibody, (Ser547)
Alternate Names
  • Transcription factor p65
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Background InformationNF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa B forms multiple homo and heterodimeric complexes, one of which is with RELA/p65. The NF-kappa-B p65 complex appears to be involved in invasin-mediated activation of IL-8 expression, as well as the inhibitor effect on I-kappa-B. Phosphorylation of p65 at serine 547 by the nuclear kinase ATM appears to repress NF-κB-dependent transcription of specific genes induced by genotoxic stress.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho NFkB p65 Antibody, (Ser547) is an antibody against phospho NFkB p65 Antibody for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human phospho NFkB p65, (Ser547).
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Chimpanzee, Mouse, Porcine, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RELA
  • NFKB3
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~62 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in p65 WT transfected U2OS cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho NFkB p65, (Ser547) in 10 µg of p65 WT transfected U2OS cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS403 04055977127355