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06-519 Anti-phospho-CREB (Ser133) Antibody

06-519
100 µL  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RWB, IH(P), IP, ChIP, Gel ElectrophoresisRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-519
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-CREB (Ser133) Antibody
Alternate Names
  • active transcription factor CREB
  • cAMP responsive element binding protein 1
  • cAMP-response element-binding protein-1
  • transactivator protein
Background InformationCREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-phospho-CREB (Ser133) Antibody (rabbit polyclonal antibody) is highly published and validated in EMSA, IHC,IHC(P), IP, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Chromatin Immunoprecipitation (ChIP)
  • Gel Electrophoresis
Application NotesImmunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected phospho CREB (Ser133) in rat cerebral cortex, human cerebral cortex, and rat hippocampus tissue.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated phospho CREB (Ser133) in 500 µg of BALB/3T3 A31+Forskolin cell lysate.
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho CREB (Ser133) in 4 µg of HEK293T cell lysate.
Gel Shift (EMSA): This antibody has been shown to gel shift by independent labs.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the KID of human phospho CREB (Ser133).
Epitopephospho-Ser133
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteMouse, Human, Rat. Predicted to react with Bovine, Canine, Porcine, Xenopus, Zebrafish based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CREB1
  • CREB
  • MGC9284
Modifications
  • Phosphorylation
Purification MethodPurified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column.
UniProt Number
Molecular Weight~43 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Forskolin treated BALB/3T3 clone A31 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho CREB (Ser133) in 10 µg of Forskolin treated BALB/3T3 clone A31 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
06-519 04053252580505