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AB9986 Anti-phospho-BRCA2 (Ser3291) Antibody

Anti-phospho-BRCA2 (Ser3291) Antibody is a rabbit polyclonal antibody for detection of phospho-BRCA2 (Ser3291), also known as BRCA1/BRCA2-containing complex subunit 2 & Fanconi anemia group D1 protein 3, has been validated in ICC, IHC, DB.

Anti-phospho-BRCA2 (Ser3291) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair, Oncoproteins Gene Symbol: BRCA2, BRCC2, BROVCA2, FACD, FAD, FAD1, FANCB, FANCD, FANCD1 UniProt Number: P51587
AB9986
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Eq, M, R, Chp, Ca, Ht, Rhesus MacaqueICC, IHC, DBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB9986
DescriptionAnti-phospho-BRCA2 (Ser3291) Antibody
Alternate Names
  • BRCA1/BRCA2-containing complex, subunit 2
  • Fanconi anemia group D1 protein 3
  • Fanconi anemia, complementation group D1
  • breast and ovarian cancer susceptibility gene, early onset
  • breast cancer 2 tumor suppressor
  • breast cancer 2, early onset
  • breast cancer susceptibility protein BRCA2
Background InformationBRCA2 (Breast Cancer Type 2 susceptibility protein) is a tumor suppressor protein encoded by the BRCA2 gene.
The protein encoded by this gene plays a direct role in error-free double-strand break repair and homologous recombination. BRCA2 shares similarities with the tumor suppressor gene BRCA1. Inherited mutations in BRCA2 as well as BRCA1 are associated with an increased risk of developing breast or ovarian cancer. Although the structures of the BRCA1 and BRCA2 genes are very different, some functions are similar. The proteins made by both genes are essential for repairing damaged DNA and interact with the protein produced by the RAD51 gene. Together these three proteins play a role in maintaining the stability of the human genome and prevent dangerous gene rearrangements that can lead to the development of cancer.
References
Product Information
FormatAffinity Purified
Control
  • Modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-BRCA2 (Ser3291) Antibody is a rabbit polyclonal antibody for detection of phospho-BRCA2 (Ser3291), also known as BRCA1/BRCA2-containing complex subunit 2 & Fanconi anemia group D1 protein 3, has been validated in ICC, IHC, DB.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Dot Blot
Application NotesImmunocytochemistry Analysis: 1:500 dilution from a previous lot detected BRCA2 in MCF-7 cells.

Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected BRCA2 in metastatic ductal carcinoma tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human BRCA2 phosphorylated at and around the internal domain surrounding Ser3291.
EpitopeInternal Domain surrounding Ser3291
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes human BRCA2 phosphorylated at and around the internal domain surrounding Ser3291.
Species Reactivity
  • Human
  • Horse
  • Mouse
  • Rat
  • Chimpanzee
  • Canine
  • Hamster
  • Rhesus Macaque
Species Reactivity NoteDemonstrated to react with human. Predicted to react with equine, mouse, rat, chimpanzee, canine, hamster, rhesus monkey, and cat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryInherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq].
Gene Symbol
  • BRCA2
  • BRCC2
  • BROVCA2
  • FACD
  • FAD
  • FAD1
  • FANCB
  • FANCD
  • FANCD1
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation.

SIZE: 3418 amino acids; 384225 Da

SUBUNIT: Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoints functions. Interacts with WDR16.

TISSUE SPECIFICITY: Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.

PTM: Phosphorylated by ATM upon irradiation-induced DNA damage.

DISEASE: Defects in BRCA2 are a cause of genetic susceptibility to breast cancer (BC) [MIM:600185, 114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. Mutations in BRCA2 are thought to be responsible for some inherited breast cancer. It is linked with male breast cancer. & Defects in BRCA2 are the cause of Fanconi anemia complementation group D type 1 (FANCD1) [MIM:605724]. Fanconi anemia [MIM:227650] is an autosomal recessive disorder affecting all bone marrow elements and associated with cardiac, renal, and limb malformations as well as dermal pigmentary changes.

SIMILARITY: Contains 8 BRCA2 repeats.
Molecular Weight~ 390 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2.

Dot Blot Analysis: 2 µg/mL of this antibody detected BRCA2 in modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB9986 04053252672262

Documentation

Anti-phospho-BRCA2 (Ser3291) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-BRCA2 (Ser3291) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-BRCA2 (Ser3291) 2482251
Anti-phospho-BRCA2 (Ser3291) - 2147099 2147099
Anti-phospho-BRCA2 (Ser3291) - 2359854 2359854
Anti-phospho-BRCA2 (Ser3291) - 2111393 2111393
Anti-phospho-BRCA2 (Ser3291) - 2513955 2513955
Anti-phospho-BRCA2 (Ser3291) - 3243471 3243471
Anti-phospho-BRCA2 (Ser3291) - NG1842845 NG1842845
Anti-phospho-BRCA2 (Ser3291) - NRG1693598 NRG1693598
Anti-phospho-BRCA2 (Ser3291) -2594279 2594279
Anti-phospho-BRCA2 (Ser3291) -2658894 2658894
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