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07-748-AF488 Anti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor® 488 conjugate

Anti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor® 488 conjugate is an antibody against monomethyl Histone H4 (Lys20) for use in Immunocytochemistry.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H4A, H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4 UniProt Number: P62805
07-748-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue Number07-748-AF488
DescriptionAnti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor® 488 conjugate
Alternate Names
  • H4K20me1
  • Histone H4, Lys20 monomethylated
  • H4 histone family member, Lys20 monomethylated
Background InformationHistone H4 (UniProt P62805; also known as H4 histone family member A, Histone 1 H4a) is encoded by the HIST1H4A (also known as H4/A, H4FA) gene (Gene ID 8359) in human. Histone H4 is one of the main histone proteins that contribut to the chromatin structure in both prokaryotic and eukaryotic cells. Consecutive methylation of the histone H4 Lys20 (H4K20) is dependent on nucleosome age, and different H4K20 methylation levels are found at distinct nuclear loci. H4K20 monomethylation (H4K20me1) is a repressive mark of transcribed genes, whereas H4K20 trimethylation (H4K20me3) is found in repetitive elements in the human genome. More than 80% of H4K20 is dimethylated in human cells. At least three different methyltransferases (KMTs) have been identified that can catalyze H4K20 methylation, while only lysine demethylases (KDMs) for H4K20me1, but not for H4K20me2 or H4K20me3, have been discovered. The monomethyltransferase gene Setd8 knockout in mouse and in Drosophila is lethal due to defects in cell cycle progression.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor® 488 conjugate is an antibody against monomethyl Histone H4 (Lys20) for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected histone H4 Lys20 monomethylation in HeLa cells.
The unconjugated antibody (Cat. No. 07-748) is shown to be suitable also for Immunocytochemistry, Multiplex assay, and Western blotting applications.
Biological Information
ImmunogenKLH-conjugated, branched synthetic peptide corresponding to amino acids 15-25 (AKRHR[Kme]VLRDN) of human Histone H4 with monomethylated Lys20.
EpitopeH4K20me1.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects histone H4 with monomethylated Lys20 (H4K20me1).
Species Reactivity
  • Human
Species Reactivity NoteHuman. Broad species cross-reactivity expected due to sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H4A
  • H4/A
  • H4FA
  • HIST1H4B
  • H4/I
  • H4FI
  • HIST1H4C
  • H4/G
  • H4FG
  • HIST1H4D
  • H4/B
  • H4FB
  • HIST1H4E
  • H4/J
  • H4FJ
  • HIST1H4F
  • H4/C
  • H4FC
  • HIST1H4H
  • H4/H
  • H4FH
  • HIST1H4I
  • H4/M
  • H4FM
  • HIST1H4J
  • H4/E
  • H4FE
  • HIST1H4K
  • H4/D
  • H4FD
  • HIST1H4L
  • H4/K
  • H4FK
  • HIST2H4A
  • H4/N
  • H4F2
  • H4FN
  • HIST2H4
  • HIST2H4B
  • H4/O
  • H4FO
  • HIST4H4
Modifications
  • Methylation
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~10 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H4 Lys20 monomethylation in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-748-AF488 04055977350647