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07-520 Anti-eNOS/NOS III Antibody, CT

07-520
200 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, B, M, Ca, Gp, PoWBRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-520
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-eNOS/NOS III Antibody, CT
OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
Alternate Names
  • Constitutive NOS
  • Endothelial NOS
  • Endothelial NOS
  • Endothelial NOS
  • endothelial nitric oxide synthase
  • nitric oxide synthase 3 (endothelial cell)
Background InformationEndothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.
Product Information
FormatPurified
Control
  • Platelets, placenta, liver and kidney.
PresentationPurified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.
Quality LevelMQ100
Applications
ApplicationAnti-eNOS/NOS III Antibody, CT detects level of eNOS/NOS III & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog.
EpitopeC-Terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes eNOS/NOS III, Mr ~135 kDa.
IsotypeIgG
Species Reactivity
  • Human
  • Rat
  • Bovine
  • Mouse
  • Canine
  • Guinea Pig
  • Pig
Species Reactivity NoteHuman and bovine.
Predicted to cross-react with mouse, guinea pig, pig, rat and dog based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOS3
  • cNOS
  • ECNOS
  • eNOS
  • EC-NOS
  • NOSIII
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
SIZE: 1203 amino acids; 133289 Da
SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain. ... hide » see more »
Molecular Weight135 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot in RIPA lysates from HUVEC cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size200 µg
Global Trade Item Number
Catalogue Number GTIN
07-520 04053252372728