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ABT1487 Anti-alpha-Actin Antibody, Smooth Muscle

Anti-alpha-Actin Antibody, Smooth Muscle is an antibody against alpha-Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation.

Anti-alpha-Actin Antibody, Smooth Muscle MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: ACTA2, AAT6, ACTSA, ACTVS, GIG46, MYMY5 UniProt Number: P62736
ABT1487
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IHC, ICC, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT1487
DescriptionAnti-alpha-Actin Antibody, Smooth Muscle
Alternate Names
  • Actin, aortic smooth muscle
  • Alpha-actin-2
  • Cell growth-inhibiting gene 46 protein
Background InformationActin, aortic smooth muscle (UniProt P62736; also known as Alpha-actin-2, Cell growth-inhibiting gene 46 protein) is encoded by the ACTA2 (also known as AAT6, ACTSA, ACTVS, GIG46, MYMY5) gene (Gene ID 59) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-alpha-Actin Antibody, Smooth Muscle is an antibody against alpha-Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunocytochemistry Analysis: 5.0 µg/mL from a representative lot immunostained smooth muscle alpha-Actin in NIH/3T3 fibroblasts, HUVECs, A431 and HeLa cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained smooth muscle cells in human uterus tissue sections.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated smooth muscle alpha-Actin from 500 µg of C2C12 mouse myoblast lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminual sequence of human smooth muscle alpha-actin.
EpitopeN-terminus.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody targets the N-terminal sequence specific to smooth muscle alpha-actin (ACTA2), but not skeletal muscle alpha-actin (ACTA1), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), or smooth muscle gamma-actin (ACTG2; ACTA3).
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Chicken based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ACTA2
  • AAT6
  • ACTSA
  • ACTVS
  • GIG46
  • MYMY5
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~42 kDa observed.
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in C2C12 mouse myoblast lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected smooth muscle alpha-Actin in 10 µg of C2C12 mouse myoblast lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Global Trade Item Number
Catalogue Number GTIN
ABT1487 04055977334241