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07-677-IS Anti-acetyl-Histone H3 (Lys56) Antibody, Trial Size

07-677-IS
20 µL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, DB, ChIPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-677-IS
DescriptionAnti-acetyl-Histone H3 (Lys56) Antibody, Trial Size
Alternate Names
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/i
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l
  • H3K56Ac
  • Histone H3 (acetyl K56)
  • Histone 3, H3
  • Histone cluster 3, H3
  • Histone H3 K56ac
  • Histone H3 K56ac
Background InformationHistone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
References
Product Information
FormatAffinity Purified
Control
  • HeLa acid extract, sodium butyrate treated HeLa acid extract, colcemid treated HeLa acid extract, recombinant Histone H3, recombinant Histone H4, and recombinant Histone H2A
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-acetyl-Histone H3 (Lys56) Antibody, Trial Size/ Pack is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys56) also known as H3K56Ac, Histone H3 (acetyl K56) & has been validated in WB, ChIP.
Key Applications
  • Western Blotting
  • Dot Blot
  • Chromatin Immunoprecipitation (ChIP)
Application NotesDot Blot (Specificity) Analysis: A representative lot detected acetyl-Histone H3 (Lys56) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667).

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys56) from HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys56.
EpitopeAcetylated Lys56
HostRabbit
SpecificityThis antibody recognizes Histone H3 acetylated at Lys56.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa acid extract, sodium butyrate treated HeLa acid extract, colcemid treated HeLa acid extract, recombinant Histone H3, recombinant Histone H4, and recombinant Histone H2A.

Western Blot Analysis: A 1:405 dilution of this antibody detected acetyl-Histone H3 (Lys56) in sodium butyrate treated HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size20 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-677-IS 04053252907517

Documentation

Anti-acetyl-Histone H3 (Lys56) Antibody, Trial Size SDS

Title

Safety Data Sheet (SDS) 

Anti-acetyl-Histone H3 (Lys56) Antibody, Trial Size Certificates of Analysis

TitleLot Number
Anti-acetyl Histone H3 (Lys56), -2621122 2621122
Anti-acetyl Histone H3 (Lys56), Trial Size 2485483
Anti-acetyl Histone H3 (Lys56), Trial Size - 3198443 3198443
Anti-acetyl Histone H3 (Lys56), Trial Size - 3387624 3387624
Anti-acetyl Histone H3 (Lys56), Trial Size - 3740967 3740967
Anti-acetyl Histone H3 (Lys56), Trial Size - Q2187997 Q2187997
Anti-acetyl Histone H3 (Lys56), Trial Size -2571262 2571262
Anti-acetyl Histone H3 (Lys56), Trial Size Polyclonal Antibody 3162897
Anti-acetyl Histone H3 (Lys56), Trial Size Polyclonal Antibody 3085010