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07-677-I Anti-acetyl-Histone H3 (Lys56) Antibody

07-677-I
200 µL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, DB, ChIPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-677-I
Replaces07-677
DescriptionAnti-acetyl-Histone H3 (Lys56) Antibody
Alternate Names
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/i
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l
  • H3K56Ac
  • Histone H3 (acetyl K56)
  • Histone H3 K56ac
Background InformationHistone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
References
Product Information
FormatAffinity Purified
Control
  • HeLa acid extract, sodium butyrate treated HeLa acid extract, colcemid treated HeLa acid extract, recombinant Histone H3, recombinant Histone H4, and recombinant Histone H2A
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-acetyl-Histone H3 (Lys56) is an antibody targeting the acetyl-Histone H3 (Lys56) protein, validated for use in WB & ChIP.
Key Applications
  • Western Blotting
  • Dot Blot
  • Chromatin Immunoprecipitation (ChIP)
Application NotesDot Blot (Specificity) Analysis: A representative lot detected acetyl-Histone H3 (Lys56) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667).

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys56) from HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys56.
EpitopeAcetylated Lys56
HostRabbit
SpecificityThis antibody recognizes Histone H3 acetylated at Lys56.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa acid extract, sodium butyrate treated HeLa acid extract, colcemid treated HeLa acid extract, recombinant Histone H3, recombinant Histone H4, and recombinant Histone H2A.

Western Blot Analysis: A 1:405 dilution of this antibody detected acetyl-Histone H3 (Lys56) in sodium butyrate treated HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-677-I 04053252828751

Documentation

Anti-acetyl-Histone H3 (Lys56) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-acetyl-Histone H3 (Lys56) Antibody Certificates of Analysis

TitleLot Number
Anti-acetyl-Histone H3 (Lys56) 2471008
Anti-acetyl-Histone H3 (Lys56) - 2379499 2379499
Anti-acetyl-Histone H3 (Lys56) - Q2112544 Q2112544
Anti-acetyl-Histone H3 (Lys56) - 2227207 2227207
Anti-acetyl-Histone H3 (Lys56) - 3270769 3270769
Anti-acetyl-Histone H3 (Lys56) - 3744328 3744328
Anti-acetyl-Histone H3 (Lys56) - 4180354 4180354
Anti-acetyl-Histone H3 (Lys56) -2514206 2514206
Anti-acetyl-Histone H3 (Lys56) -2574090 2574090
Anti-acetyl-Histone H3 (Lys56) -2601732 2601732

Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics