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ABN1452 Anti-TRIO

Anti-TRIO Antibody, Cat. No. ABN1452, is a highly specific rabbit polyclonal antibody that targets Triple functional domain protein (TRIO) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-TRIO MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: TRIO UniProt Number: O75962
ABN1452
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1452
DescriptionAnti-TRIO
Alternate Names
  • Triple functional domain protein
  • EC: 2.7.11.1.
Background InformationTriple functional domain protein (UniProt: O75962; also known as EC: 2.7.11.1, PTPRF-interacting protein, TRIO) is encoded by the TRIO gene (Gene ID: 7204) in human. TRIO is a complex protein that is widely expressed in human tissue, with highest levels reported in heart, skeletal muscle, and brain. Five isoforms of TRIO have been reported that are produced by alternative splicing. It contains three catalytic domains, two tandem Dbl Homology (DH) and pleckstrin homology (PH) domains, and a serine/Threonine kinase domain. The DH-PH module is involved in guanine nucleotide exchange and the activation of the small GTPases of the Rho family. The DH1-PH1 domain (GEF1) is reported to activate the Rho G and Rac1, whereas DH2-PH2 (GEF2) is involved in the activation of RhoA. TRIO is involved in coordinating actin remodeling, which is necessary for cell migration and growth. In developing hippocampal neurons it limits dendrite formation, without affecting the establishment of axon polarity. After dendrites are formed, it is reported to be involved in the control of synaptic function by regulating the endocytosis of AMPA-selective glutamate receptors at CA1 excitatory synapses. Mutations in TRIO gene have been linked to mental retardation and microcephaly. (Ref.: Debant, A. et al. (1996). Proc. Natl. Acad. Sci, USA. 93(11); 5466-71; Bellanger, JM. Et al. (2000). Nat. Cell Biol. 2(12); 888-92).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TRIO Antibody, Cat. No. ABN1452, is a highly specific rabbit polyclonal antibody that targets Triple functional domain protein (TRIO) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution of this antibody detected TRIO in human cerebral cortex, human pancreas, and human testis tissue.
Biological Information
ImmunogenKLH-conjugated liner peptide corresponding to 17 amino acids from the C-terminal half of human Triple functional domain protein.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects human Triple functional domain protein (TRIO). It targets an epitope within 17 amino acids from the C-terminal half. The immunogen sequence is conserved in all 5 isoforms.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TRIO
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~300 kDa observed; 346.90 kDa calculalted. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TRIO in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1452 04054839396519