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ABN1471 Anti-TREM-2

Anti-TREM-2, Cat. No. ABN1471, is a highly specific rabbit polyclonal antibody that targets Triggering receptor expressed on myeloid cells 2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-TREM-2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: TREM2 UniProt Number: Q9NZC2
ABN1471
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1471
DescriptionAnti-TREM-2
Alternate Names
  • Triggering receptor expressed on myeloid cells 2
  • Triggering receptor expressed on monocytes 2
Background InformationTriggering receptor expressed on myeloid cells 2 (UniProt: Q9NZC2; also known as TREM-2, Triggering receptor expressed on monocytes 2) is encoded by the TREM2 gene (Gene ID: 54209) in human. TREM2 is an Ig-like V-type receptor expressed by populations of myeloid cells in various region of the central nervous system. Its expression is also reported on macrophages and dendritic cells, but not on granulocytes or monocytes. TREM2 is highly expressed in microglia, where it, along with its adaptor protein DAP12, regulates inflammatory cytokine release and phagocytosis of apoptotic neurons. TREM-2 plays a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. It forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Apolipoprotein E (ApoE) is reported to function as a high-affinity ligand for TREM-2. ApoE binding is shown to enhance the phagocytosis of apoptotic N2a cells by primary microglia cells. Greatly reduced ApoE binding is seen with Alzheimer s disease-associated TREM2 R47H mutant. TREM2 is produced with a signal peptide sequence (a.a. 1-18), the removal of which yields the mature single-transmembrane (a.a. 175-195) protein with a large extracellular region (a.a. 19-174) that contains the V-type Ig-like domain (a.a. 29-112) and a cytoplasmic tail (a.a. 196-230). The short cytoplasmic tail contains no signaling motifs and relies on its association with the immunoreceptor tyrosine-based activation motif- (ITAM-) containing TYROBP for signal transduction.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TREM-2, Cat. No. ABN1471, is a highly specific rabbit polyclonal antibody that targets Triggering receptor expressed on myeloid cells 2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TREM-2 in human cerebellum, human bone marrow, human colon, and human kidney tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human TREM-2.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody antibody detects Triggering receptor expressed on myeloid cells 2 in several regions of human brain. It targets an epitope within 13 amino acids from the C-terminal region.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TREM2
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~27 kDa observed; 25.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human cerebellum tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected TREM-2 in 10 µg of human cerebellum tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1471 04054839201349