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ABS1489 Anti-TRAP-1 (HSP75)

Anti-TRAP-1 (HSP75), Cat. No. ABS1489, is a rabbit polyclonal antibody that detects TRAP-1 (HSP75) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-TRAP-1 (HSP75) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Trap1, Hsp75 UniProt Number: Q12931
ABS1489
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1489
DescriptionAnti-TRAP-1 (HSP75)
Alternate Names
  • Heat shock protein 75 kDa mitochondrial
  • TNFR-associated protein 1
  • Tumor necrosis factor type 1 receptor-associated protein
  • TRAP-1
Background InformationHeat shock protein 75 kDa, mitochondrial (UniProt: Q12931; also known as HSP 75, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein, TRAP-1) is encoded by the Trap1 (also known as Hsp75) gene (Gene ID: 287069) in human. TRAP-1 is a mitochondrial protein that serves as a chaperone with ATPase activity. Its ATPase activity is inhibited by small molecule pocket antagonists, such as geldanamycin or radicicol. TRAP-1 is expressed in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Higher expression levels are observed in several cancer cell lines. However, bladder and renal cancers display reduced levels of TRAP-1. TRAP-1 is involved in maintaining mitochondrial function and polarization, likely through stabilization of mitochondrial complex I. Two isoforms of TRAP-1 have been reported that are produced by alternative splicing. It is synthesized with a transit peptide (aa 1-50) that is subsequently cleaved off. TRAP-1 is reported to protect cells against mitochondrial apoptosis and helps cells to cope with oxidative stress. (Ref.: Altieri, DC et al. (2012). Biochim. Biophys. Acta 1823(3); 767-773).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TRAP-1 (HSP75), Cat. No. ABS1489, is a rabbit polyclonal antibody that detects TRAP-1 (HSP75) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected TRAP-1 (HSP75) in 10 µg of HeLa cell lysate.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected TRAP-1 (HSP75) in human brain, human tonsil, and human prostate cancer tissues.
Biological Information
ImmunogenKLH-conjugated linenar peptide corresponding to 12 amino acids from the C-terminal half of human TRAP-1 (HSP75). The immunogen sequence is conserved in both isoforms.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Tumor necrosis factor type 1 receptor-associated protein (TRAP-1; HSP75) in human cells. It targets an epitope within 12 amino acids from the C-terminal half.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Trap1
  • Hsp75
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~75 kDa observed; 80.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TRAP-1 (HSP75) in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1489 04054839402111