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MABN894 Anti-Synapsin-1 Antibody, clone 10.22

This Anti-Synapsin-1 Antibody, clone 10.22 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, Immunofluorescence for the detection of Synapsin-1.

Anti-Synapsin-1 Antibody, clone 10.22 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: SYN1 UniProt Number: P17599
MABN894
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, M, B, FWB, IH(P), IP, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN894
DescriptionAnti-Synapsin-1 Antibody, clone 10.22
Alternate Names
  • Synapsin I
  • Synapsin-1
Background InformationSynapsin-1 (UniProt P17599; also known as Synapsin I) is encoded by the SYN1 gene (Gene ID 281510) in bovine species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1–2 months of life. The NH2-terminal region is divided into A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The C-terminal region contains spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn IIa and IIb, domain H in Syn IIa, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Synapsin-1 Antibody, clone 10.22 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, Immunofluorescence for the detection of Synapsin-1.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synapsin-1 in human, mouse, and rat cerebral cortex tissues.
Western Blotting Analysis: A representative lot detected synapsin Ia/Ib in mouse cortical neuron lysates (Medrihan, L., et al. (2013). Nat. Commun. 4:1512).
Western Blotting Analysis: A representative lot detected purified bovine brain synapsin Ia/Ib (Messa, M., et al. (2010). J. Cell Sci. 123(13):2256-2265).
Western Blotting Analysis: A representative lot detected synapsin-1 in Torpedo (electric ray) synaptosomal preparations and in GST-cyclophilin B pull-downs (Lane-Guermonprez, L., et al. (2005). J. Neurochem. 93(6):1401-1411).
Western Blotting Analysis: A representative lot detected synapsin-1 in the same rat brain subcellular fractions as cyclophilin B (Lane-Guermonprez, L., et al. (2005). J. Neurochem. 93(6):1401-1411).
Western Blotting Analysis: A representative lot detected synapsin Ia/Ib, but not IIa/IIb, in rat brain post-nuclear supernatants (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Immunoprecipitation Analysis: A representative lot immunoprecipitated synapsin Ia/Ib, but not IIa/IIb, from rat brain synaptosomal preparations using protein G beads with pre-bound rabbit anti-mouse IgG (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Immunofluorescence Analysis: Clone 10.22 ascites fluid was employed to localize synapsin-1 immunoreactivity within retinal inner plexiform layer (IPL) of floating or whole-mount rat retinas (Mandell, J.W., et al. (1992). J. Neurosci. 12(5):1736-1749).
Note: Clone 10.22 does not bind significantly to protein G. For immunoprecipitation application, pre-coat protein G beads with an anti-mouse IgG antibody is recommended (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Biological Information
ImmunogenPurified bovine brain synapsin-1.
EpitopePro-rich domain D.
Clone10.22
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 10.22 reacts with both synapsin-1 spliced isoforms (Ia and Ib), but not synapsin-2 spliced isoform IIa or IIb (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
IsotypeIgG1
Species Reactivity
  • Rat
  • Human
  • Mouse
  • Bovine
  • Fish
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SYN1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~75 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain cytosol tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Synapsin-1 in 10 µg of rat brain cytosol tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN894 04055977312935

Documentation

Anti-Synapsin-1 Antibody, clone 10.22 SDS

Title

Safety Data Sheet (SDS) 

Anti-Synapsin-1 Antibody, clone 10.22 Certificates of Analysis

TitleLot Number
Anti-Synapsin-1, clone 10.22 - 3176776 3176776
Anti-Synapsin-1, clone 10.22 - 3384839 3384839
Anti-Synapsin-1, clone 10.22 - 4020569 4020569
Anti-Synapsin-1, clone 10.22 - 4158974 4158974
Anti-Synapsin-1, clone 10.22 - 4168795 4168795
Anti-Synapsin-1, clone 10.22 -2786918 2786918
Anti-Synapsin-1, clone 10.22 -Q2640160 Q2640160
Anti-Synapsin-1, clone 10.22 Monoclonal Antibody 3002735

Brochure

Title
Neuroscience Solutions for productive research