Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

DP02A Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate

This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate is validated for use in Immunoprecipitation, Affinity Purification for the detection of SV40 T Antigen (Ab-2).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
DP02A
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Sv40 Infected CellsMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DP02A-0.5ML Glass bottle 0.5 ml
Description
OverviewRecognizes the ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells. Cat. No. DP02A is linked to agarose for immunoprecipitation and purification.
Catalogue NumberDP02A
Brand Family Calbiochem®
References
ReferencesDeCaprio, J.A., et al. 1988. Cell 54, 275.
Whyte, P., et al. 1988. Nature 334, 124.
Mitchell, P.J., et al. 1987. Cell 50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
Simanis, V. and Lane, D.P., 1985. Virol. 144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379
Sarnow, P., et al. 1982. Cell 28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
Tooze, J.1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol. 10,
Product Information
FormLiquid slurry
Formulation50% slurry in PBS.
Positive controlSV80 or SV-T2 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Harlow, E., et al. 1981. J. Virol. 39, 861.
Key Applications Affinity Purification
Immunoprecipitation
Application NotesImmunoprecipitation (15 µl suspension/sample)
Affinity Purification (see comments)
Application CommentsSuitable for affinity purification and immunoprecipitation. The amount of suspension required for purification is dependent on individual sample types.
Biological Information
Immunogenpurified SV40 large T-antigen
EpitopeWithin amino acids 83-128
ClonePAb416
HostMouse
IsotypeIgG2a
Species Reactivity
  • Sv40 Infected Cells
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DP02A-0.5ML 04055977226553

Documentation

Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate Certificates of Analysis

TitleLot Number
DP02A

References

Reference overview
DeCaprio, J.A., et al. 1988. Cell 54, 275.
Whyte, P., et al. 1988. Nature 334, 124.
Mitchell, P.J., et al. 1987. Cell 50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
Simanis, V. and Lane, D.P., 1985. Virol. 144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379
Sarnow, P., et al. 1982. Cell 28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
Tooze, J.1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol. 10,
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
ApplicationImmunoprecipitation (15 µl suspension/sample)
Affinity Purification (see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Supplied as an agarose conjugate. Recognizes the ~94 kDa SV40 large T antigen.
BackgroundSV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
HostMouse
Immunogenpurified SV40 large T-antigen
EpitopeWithin amino acids 83-128
ClonePAb416
IsotypeIgG2a
SpeciesSV40 infected cells
Positive controlSV80 or SV-T2 cells
FormLiquid slurry
Formulation50% slurry in PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsSuitable for affinity purification and immunoprecipitation. The amount of suspension required for purification is dependent on individual sample types.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesDeCaprio, J.A., et al. 1988. Cell 54, 275.
Whyte, P., et al. 1988. Nature 334, 124.
Mitchell, P.J., et al. 1987. Cell 50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
Simanis, V. and Lane, D.P., 1985. Virol. 144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379
Sarnow, P., et al. 1982. Cell 28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
Tooze, J.1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol. 10,
Application referencesOriginal Clone Harlow, E., et al. 1981. J. Virol. 39, 861.