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MABF2155-25UG Anti-Ricin A Chain Antibody, clone RAC18

Anti-Ricin A Chain, clone RAC18, Cat. No. MABF2155, is a mouse monoclonal antibody that detects Ricin A chain and has been tested for use in ELISA, Inhibition Assay, and Neutralizing.

MABF2155-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2155-25UG
DescriptionAnti-Ricin A Chain Antibody, clone RAC18
Alternate Names
  • RAC
Background InformationRicin is a dimeric toxin derived from Ricinus communis (Caster bean). It is composed of a sugar-binding subunit (B chain) that attached to cell surface receptors and facilitate the entry of toxin into the target cell and a second subunit (A chain), whcih has glycosidase activity that inactivates ribosomes by depurinating a single adenosine residue from an exposed loop of the 28S ribosomal RNA. This leads to inhibition of protein synthesis in cells. The toxic effects of Ricin are attributed to the A chain. Clone RAC18 displays high avidity for intact ricin and inhibits its activity without affecting binding of ricin to cells. Immunization with RAC18 is shown to offer significant protection against ricin toxicity. (Ref.: Maddaloni, M., et al. (2004). J. Immunol. 172; 6221-6228).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS without preservatives.
Applications
ApplicationAnti-Ricin A Chain, clone RAC18, Cat. No. MABF2155, is a mouse monoclonal antibody that detects Ricin A chain and has been tested for use in ELISA, Inhibition Assay, and Neutralizing.
Key Applications
  • ELISA
  • Inhibition
  • Neutralizing
Application NotesNeutralizing Analysis: A representative lot neutralized the activity of Ricin A chain. (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8).

ELISA Analysis: A representative lot detected Ricin A chain in ELISA applications (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8; Simon, S., et. al. (2015). Toxins (Basel) 7(12):4967-86).

Inhibition Analysis: A representative lot inhibited the enzymatic activity of Ricin. (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8).
Biological Information
ImmunogenPurified Ricin A chain.
CloneRAC18
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone RAC18 bind to Ricin A chain
IsotypeIgG2aκ
Species Reactivity
  • Ricinus communis
Species Reactivity NoteRicinus communis.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Molecular Weight29.89 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with Ricin A chain.

ELISA Analysis: Various dilutions of this antibody detected Ricin A chain in an indirect ELISA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2155-25UG 04054839660016