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16-321 Anti-Ras Magnetic Bead Conjugate Antibody

Anti-Ras Magnetic Bead Conjugate Antibody detects level of Ras Magnetic Bead Conjugate & has been published & validated for use in WB & IP.

Anti-Ras Magnetic Bead Conjugate Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases, G-proteins Gene Symbol: K-Ras, Ki-Ras, K-Ras2, Kras-2, p21B, KRAS, RASK2, HRAS, HA_RAS, N-RAS, H-RAS, NRAS, NRAS1, ALPS4 UniProt Number: P01116
16-321
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, IPMPurifiedMonoclonal Antibody
Description
Catalogue Number16-321
DescriptionAnti-Ras Magnetic Bead Conjugate Antibody
Alternate Names
  • GTP- and GDP-binding peptide B
  • GTPase Hras
  • Ha-Ras1 proto-oncoprotein
  • Ras family small GTP binding protein H-Ras
  • Transforming protein p2
  • c-has/bas p21 protein
  • c-ras-Ki-2 activated oncogene
  • p19 H-RasIDX protein
  • transformation gene: oncogene HAMSV
  • v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Background InformationThe tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.
Product Information
FormatPurified
Control
  • A431 whole cell lysate
Presentation20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 immunoprecipitation assays.
Quality LevelMQ100
Applications
ApplicationAnti-Ras Magnetic Bead Conjugate Antibody detects level of Ras Magnetic Bead Conjugate & has been published & validated for use in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation:
150 μg of this antibody has been reported to immunoprecipitate Ras.
Biological Information
ImmunogenKLH-conjugated protein corresponding to human Ras.
HostMouse
SpecificityThis antibody recognizes p21H-, K- and N-Ras.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteProven to react with human, mouse and rat. Other species unknown.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryMembers of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM]
Gene Symbol
  • K-Ras
  • Ki-Ras
  • K-Ras2
  • Kras-2
  • p21B
  • KRAS
  • RASK2
  • HRAS
  • HA_RAS
  • N-RAS
  • H-RAS
  • NRAS
  • NRAS1
  • ALPS4
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
SIZE: 189 amino acids; 21,656 Da
SUBUNIT: Interacts with PHLPP (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side.
Involvement in disease:
Defects in KRAS are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.

Defects in KRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.

Defects in KRAS are the cause of Noonan syndrome 3 (NS3) [MIM:609942]. Noonan syndrome (NS) [MIM:163950] is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS3 inheritance is autosomal dominant.

Defects in KRAS are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.

KRAS mutations are involved in cancer development.
Molecular Weight21 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
DO NOT FREEZE
Packaging Information
Material Size200 µL
Global Trade Item Number
Catalogue Number GTIN
16-321 04053252751271