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07-1464 Anti-Rac1 Antibody

Anti-Rac1, Cat. No. 07-1464, is a rabbit polyclonal antibody that detects Rac1 and is tested for use in Peptide Inhibition Assay and Western Blotting.

Anti-Rac1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: RAC1, ORFNames=MIG5, TC-25, p21-Rac1, MGC111543, PAK1, MIG5 UniProt Number: P63000
07-1464
100 µg  
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Overview

Replacement Information
Replacement Information07-1464 is a recommended replacement for AB4202

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, PIARbPurifiedPolyclonal Antibody
Description
Catalogue Number07-1464
ReplacesAB4202
DescriptionAnti-Rac1 Antibody
Alternate Names
  • Ras-related C3 Botulinum Toxin Substrate 1
  • p21-Rac1
  • Ras-like Protein TC25
Background InformationRas-related C3 botulinum toxin substrate 1 (UniProt: P63000; also known as EC:3.6.5.2, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1) is encoded by the RAC1 (also known as TC25, MIG5) gene (Gene ID: 5879) in human. Rac1 is a plasma membrane-associated small GTPase that cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses. Two isoforms of Rac1 have been described that are produced by alternative splicing. Rac1b differs from Rac1a in that it has an additional 19 amino acids and is found in various tumors, such as breasts and colorectal. Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It can bind to the GTPase-binding domain of PAK, but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Rac1b is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. Rac1 is shown to participate in the tumor cell cycle, apoptosis, proliferation, invasion, migration, and angiogenesis, and in the regulation of tumor stem cell, thus promoting the occurrence of tumors. However, it also plays a key role in anti-tumor therapy and participates in immune escape mediated by the tumor microenvironment. (Ref.: Liang, J., et al. (2021). Front. Oncol. 11; 674426; Fiegen, D., et al. (2004). J. Biol. Chem. 279(6); 4743-4749).
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation
    Immunohistochemistry: Rat brain sections
PresentationPurified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-Rac1, Cat. No. 07-1464, is a rabbit polyclonal antibody that detects Rac1 and is tested for use in Peptide Inhibition Assay and Western Blotting.
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesTested applications

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Rac1 in Rat Brain Membrane preparations and in lysates from L6, A431, and NIH3T3 cells.

Peptide Inhibition Assay: Target band detection in lysate from Rat brain tissue was prevented by preblocking of a representative lot with the immunogen peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal half of human Rac1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Rac1 protein. It targets an epitope within 12 amino acids from the C-terminal half.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]
Gene Symbol
  • RAC1
  • ORFNames=MIG5
  • TC-25
  • p21-Rac1
  • MGC111543
  • PAK1
  • MIG5
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
FUNCTION: Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.
SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with NISCH (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
Molecular Weight~21 kDa observed; 21.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Rac1 in Rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-1464 04053252328893

Documentation

Anti-Rac1 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Rac1 Antibody Certificates of Analysis

TitleLot Number
Anti-Rac1 - 2110564 2110564
Anti-Rac1 - 2390456 2390456
Anti-Rac1 - 2012975 2012975
Anti-Rac1 - 2147137 2147137
Anti-Rac1 - 2294371 2294371
Anti-Rac1 - 2488837 2488837
Anti-Rac1 - 3307794 3307794
Anti-Rac1 - 3517522 3517522
Anti-Rac1 - 3666838 3666838
Anti-Rac1 - 3698139 3698139
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References

Reference overviewApplicationPub Med ID
The matricellular protein CCN1 induces fibroblast senescence and restricts fibrosis in cutaneous wound healing.
Jun, JI; Lau, LF
Nature cell biology  12  676-85  2010

Show Abstract 		Western Blotting20526329 20526329