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06-1028 Anti-Probable ergosterol biosynthetic protein 28 Antibody

06-1028
200 µL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Rhesus MacaqueWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-1028
DescriptionAnti-Probable ergosterol biosynthetic protein 28 Antibody
Alternate Names
  • Probable ergosterol biosynthetic protein 28
  • NET51
Background InformationProbable ergosterol biosynthetic protein 28, also known as NET51, is a very basic polypeptide containing several, evolutionary conserved transmembrane domains that are likely to play a role in sterol biosynthesis. It is found predominantly in normal testis and has also been found in many cancer cell lines and tumors.
References
Product Information
FormatAffinity Purified
Control
  • Human testis tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Probable ergosterol biosynthetic protein 28 Antibody is validated for use in WB, IC for the detection of Probable ergosterol biosynthetic protein 28.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected Probable ergosterol biosynthetic protein 28 in HeLa cells.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in rat liver cells. (Malik, P., et al. (2010). Cell. Mol. Life Sci. DOI 10.1007/s00018-010-0257-2.)
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Probable ergosterol biosynthetic protein 28 near the first transmembrane domain.
EpitopeTransmembrane domain
HostRabbit
SpecificityThis antibody recognizes Probable ergosterol biosynthetic protein 28 near the first transmembrane domain.
Species Reactivity
  • Human
  • Rhesus Macaque
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Rhesus Monkey, Orangutan, Rabbit, and Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NET51
  • C14orf1
Purification MethodAffinity Purfied
UniProt Number
UniProt SummarySUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential).

TISSUE SPECIFICTY: Ubiquitous; strongly expressed in testis and some cancer cell lines.

SEQUENCE SIMILARITIES: Belongs to the ERG28 family.

SEQUENCE CAUTION: The sequence AAD51373.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAF28966.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight~16 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 1:220 dilution of this antibody detected Probable ergosterol biosynthetic protein 28 on 10 µg of human testis tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
06-1028 04053252555817

Documentation

Anti-Probable ergosterol biosynthetic protein 28 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Probable ergosterol biosynthetic protein 28 Antibody Certificates of Analysis

TitleLot Number
Anti-Probable ergosterol biosynthetic -2798297 2798297
Anti-Probable ergosterol biosynthetic protein 28 - NRG1878272 NRG1878272
Anti-Probable ergosterol biosynthetic protein 28 -2722593 2722593
Anti-Probable ergosterol biosynthetic protein 28 -2759638 2759638

Brochure

Title
New Products: Volume 3, 2012