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16-454 Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate

16-454
10 blots  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, ELISAMHRPMonoclonal Antibody
Description
Catalogue Number16-454
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate
Alternate Names
  • PY20
  • anti-phosphotyrosine antibody
Background InformationProtein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10® monoclonal anti-phosphotyrosine antibody (Cat. # 05-321) has been widely recognized as a highly specific and sensitive. This antibody is suitable for all applications involving protein tyrosine phosphorylation. The slightly less sensitive, but still highly regarded clone PY20 can be used as an alternative to the 4G10® product line.
Product Information
FormatHRP
Presentation100 μL of protein A purified mouse IgG2b in PBS containing 0.05% Kathon® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.
Quality LevelMQ100
Applications
ApplicationAnti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate is a Mouse Monoclonal Antibody for the detection of Phosphotyrosine & has been validated in WB, ELISA.
Key Applications
  • Western Blotting
  • ELISA
Biological Information
ImmunogenPhosphotyrosine conjugated to carrier protein
Epitopephosphotyrosine
ClonePY20
HostMouse
IsotypeIgG2b
Species Reactivity
  • All
Species Reactivity NoteRecognizes proteins containing phosphotyrosine residues, regardless of origin.
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein A Purfied
Molecular WeightVaries
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size10 blots
Global Trade Item Number
Catalogue Number GTIN
16-454 04053252327643